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Caspian Journal of Enviromental Sciences، جلد ۲۳، شماره ۲، صفحات ۳۵۵-۳۶۷
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عنوان انگلیسی Comprehensive molecular genetic characterization of the ICE1 gene in Arum korolkowii and functional insights into its encoded protein
چکیده انگلیسی مقاله A novel gene, AkICE1, and the protein it encodes were molecularly and genetically characterized from Arum korolkowii, a rare endemic species listed in Kazakhstan's Red Book. This plant faces increasing threats from environmental stress, particularly cold stress, exacerbated by climate change. Understanding the genetic mechanisms that underlie cold stress tolerance in such endemic species is critical for conservation and biodiversity preservation. The complete sequence of the AkICE1 gene (2847 bp) was obtained and deposited in GenBank (accession number OR736143). The gene comprises three exons and two introns, encoding a protein of 545 amino acids. Bioinformatic analysis revealed that AkICE1 shares conserved domains with other ICE1 proteins, such as the MYC-like basic helix-loop-helix (bHLH) domain, a serine-rich region (S-rich), and a zipper region (ZIP), all of which are typical of cold stress-related transcription factors. The protein also contains a nuclear localization signal (NLS), suggesting its role in the nucleus as a transcription factor. Additionally, the presence of a sumoylation site, which is crucial for the activation and stability of ICE1 proteins, was identified in AkICE1, indicating that its activity may be regulated by SUMO E3 ligase. The structural analysis predicted 8 alpha helices, 3 beta turns, and 11 coils in the protein, with phosphorylation sites for serine, threonine, and tyrosine residues, further supporting its involvement in cold stress regulation. Phylogenetic analysis revealed that AkICE1 shares the highest sequence similarity with the ICE1 gene of Colocasia esculenta, highlighting its functional conservation within the Aroid family. These findings establish AkICE1 as a novel ICE1-like transcription factor that likely plays a crucial role in cold stress adaptation in A. korolkowii. The study contributes to understanding the molecular mechanisms of cold stress regulation in endemic species, which can aid in developing conservation strategies and breeding programs to improve the resilience of A. korolkowii and other endemic plants to environmental stresses.
کلیدواژه‌های انگلیسی مقاله AkICE1,Cold stress,Molecular characterization,Endemic species,Transcription factor
نویسندگان مقاله Aliya Nagiyeva |
Zhangir Khan West Kazakhstan University, Kazakhstan

Gulzhaina Alpamyssova |
Department of Biotechnology, M. Auezov South Kazakhstan University, Shymkent 160000, Kazakhstan

Yakub Ametov |
Karakalpak State University named after Berdakh, Faculty of Biology, Nukus, 230112, Uzbekistan

Hamdam Tursunboev |
Karakalpak State University named after Berdakh, Faculty of Biology, Nukus, 230112, Uzbekistan

Maxmut Allamuratov |
Karakalpak State University named after Berdakh, Faculty of Biology, Nukus, 230112, Uzbekistan

Zukhra Aytmuratova |
Karakalpak State University named after Berdakh, Faculty of Biology, Nukus, 230112, Uzbekistan

Akmaral Alpisbaeva |
Karakalpak State University named after Berdakh, Faculty of Biology, Nukus, 230112, Uzbekistan

Dilfuza Bekniyazova |
Karakalpak State University named after Berdakh, Faculty of Biology, Nukus, 230112, Uzbekistan

Aigerim Yeginbay |
Department of Biotechnology, M. Auezov South Kazakhstan University, Shymkent 160000, Kazakhstan

Assilbek Burabaev |
3South Kazakhstan Medical Academy, Shymkent 160000, Kazakhstan

Altunbek Burabaev |
South Kazakhstan Medical Academy, Shymkent 160000, Kazakhstan

نشانی اینترنتی https://cjes.guilan.ac.ir/article_8647_9ef751e23d94265ab8d06f7a1c7551fb.pdf
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