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Caspian Journal of Enviromental Sciences، جلد ۲۳، شماره ۲، صفحات ۳۷۷-۳۸۳
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عنوان انگلیسی Extraction of phytoecdysteroids from the endemic medicinal plant, Silene brahuica Boiss.
چکیده انگلیسی مقاله Indigenous plant drugs are a significant contributor to the developmental process of natural drugs as a rich source of bioactive metabolites. Isolation and characterization of phytoecdysteroids from the endemic species, Silene brahuica found in Kazakhstan were carried out keeping in mind the estimation of compounds concentrations and determination of their medical potential. After ethanol extraction from the aerial parts of the plant (extract yield: 12.5% ​​w/w), purification was conducted with silica gel column chromatography and advanced HPLC. Identification by NMR and LC-MS/MS spectroscopy of three major phytoecdysteroid compounds, i.e., 20-hydroxyecdysone (20E) at a level of 2.3 mg g-1 dry weight, polypodin B (0.8 mg g-1) and ajusteroid A (0.5 mg g-1), was confirmed by the findings. Comparison with other species of Silene revealed that S. brahuica had a 30-50% greater amount of 20E than the worldwide average. Bioassays also showed significant anti-inflammatory activity (IC50: 18.5 μg mL-1 in inhibition of COX-2) and significant antioxidant activity (EC50: 42.3 μg mL-1 in DPPH test). The overall purity efficiency was found to be 85% and the final purity of the compounds was ≥ 98.7%. These findings point to the excellent potential of S. brahuica as a prospective source for the production of ecdysteroid-derived medicines and the necessity of protecting the endemic Kazakhstani genetic pool.
 
کلیدواژه‌های انگلیسی مقاله Phytoecdysteroids,Silene brahuica,Quantification,20-hydroxyecdysone,Anti-inflammatory activity,Kazakhstan
نویسندگان مقاله Marat Tynykulov |
Department of Biotechnology and Microbiology, Faculty of Natural Sciences, L.N. Gumilyov Eurasian National University, Astana, Kazakhstan

Anna Kornilova |
Department of Biology, Faculty of Mathematics and Natural Sciences, M. Kozybaev North Kazakhstan State University, Petropavlovsk, Kazakhstan

Rimma Kozhagaliyeva |
Head of educational programs for the training of teachers of biology, geography, chemistry, Faculty of Natural Geography, Department of Biology and Ecology, M. Utemisov West Kazakhstan University, Uralsk, Kazakhstan

Samalbek Kossanov |
Botanical Garden and Greenhouse, Department of Biology, Geography and Chemistry, Korkyt ata Kyzylorda State University, Kyzylorda, Kazakhstan

Gulmira Kyzdarbekova |
Department of Biology and Teaching Methods, Pedagogical Institute, Sh. Ualikhanov Kokshetau University, Kokshetau, Kazakhstan

Saule Bazarbaeva |
Department of Biology, Faculty of Mathematics and Natural Sciences, M. Kozybaev North Kazakhstan State University, Petropavlovsk, Kazakhstan

Zhannar Gumarova |
Department of Industrial Ecology, Institute of Veterinary and Agricultural Technologies, Zhangir Khan West- Kazakhstan Agrarian and Technical University, Uralsk, Kazakhstan

Murat Nurushev |
Higher School of Natural Sciences, Astana International University, Astana, Kazakhstan

نشانی اینترنتی https://cjes.guilan.ac.ir/article_8687_a6d8fde644b919bf08e70cbb5b349831.pdf
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