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Iranian Journal of Basic Medical Sciences، جلد ۲۸، شماره ۸، صفحات ۱۱۰۰-۱۱۰۶
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عنوان انگلیسی Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury
چکیده انگلیسی مقاله Objective(s): Acetaminophen (APAP)-mediated liver injury poses a significant public health concern. Curcuma xanthorrhiza extract (CXE) has been traditionally used for its hepatoprotective properties. This research aimed to assess the hepatoprotective effects of CXE in APAP-mediated hepatotoxicity by investigating the modulatory effects of CXE on key biomarkers, including Interleukin (IL), namely, (IL-6), IL-10, IL-1β, Nitric Oxide (NO), Lactate Dehydrogenase (LDH), and the genes expression related to apoptosis-like Caspase-3 (Casp-3), Casp-9, and genes related to liver metabolic c-Jun N-terminal Kinase (JNK), in APAP-mediated HepG2 cells.Materials and Methods: APAP-induced HepG2 cells were treated with different concentrations of CXE. IL-6, IL-10, IL were measured using an Enzyme-linked Immunosorbent Assay (ELISA) and NO, LDH were measured using colorimetric assay. Gene expression was analyzed using quantitative Real-Time Reverse Transcription (qRT-PCR).Results: CXE significantly reduced IL-1β and IL-6 levels, enhanced IL-10 production, and attenuated NO levels in APAP-mediated hepatotoxicity. CXE also suppressed the expression of Casp-9, Casp-3, JNK, and LDH levels. The study presented a concentration-dependent response, with 125 μg/ml CXE exhibiting the most pronounced effects. CXE effectively modulated immune responses, decreased oxidative stress, and inhibited apoptotic and inflammatory pathways in APAP-mediated hepatotoxic cells.Conclusion: These studies highlight the CXE potential as a therapeutic candidate for liver disorders, particularly in drug-mediated liver injury.
کلیدواژه‌های انگلیسی مقاله Acetaminophen, Antioxidants, Apoptosis, Curcuma xanthorrhiza, Free radical scavengers Liver injury
نویسندگان مقاله | I Nyoman Lister
Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia


| Linda Chiuman
Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia


| Maya Mutia
Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia


| Hartono Hartono
Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia


| Ermi Girsang
Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia


| Annisa Sutendi
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia


| Hanna Kusuma
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia


| Dhanar Hadiprasetyo
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia|Faculty of Pharmacy, Jenderal Achmad Yani University, Cimahi 40531, Indonesia


| Wahyu Widowati
Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia
نشانی اینترنتی https://ijbms.mums.ac.ir/article_26052.html
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