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Iranian Journal of Microbiology، جلد ۱۷، شماره ۳، صفحات ۴۸۸-۵۰۲
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عنوان انگلیسی Design and comparison of PCR-ELISA reaction with other available hybridization methods to identify types 11, 16, and 18 of the human papillomavirus
چکیده انگلیسی مقاله Background and Objectives: Human papillomavirus (HPV) is a significant etiological agent in cervical cancer. This study aimed to evaluate the performance of PCR-ELISA for detecting HPV genotypes 11, 16, and 18 compared to the conventional hybridization methods. Materials and Methods: PCR-ELISA was designed and optimized to detect target HPV genotypes using biotin-labeled probes. Sensitivity, specificity and reproducibility were assessed through intra-assay and inter-assay variability tests. Additionally, a cost-benefit analysis was performed to compare PCR-ELISA with RT-PCR and gel electrophoresis. Results: PCR-ELISA demonstrated high sensitivity (HPV18: 94.92%, HPV16: 98.36%, HPV11: 93.75%) and specificity (100% for all genotypes), with Kappa values ranging from 0.84 to 0.92, indicating strong agreement with the reference standard. Reproducibility analysis showed intra-assay CVs below 5% for most samples and inter-assay CVs within acceptable limits. The cost-benefit analysis revealed significant reductions in reagent and equipment costs compared to RT-PCR, making PCR-ELISA a cost-effective alternative. Conclusion: PCR-ELISA offers a reliable, sensitive and cost-effective method for HPV detection, particularly in resource-limited settings. Its simplicity and compatibility with existing workflows makes it a promising tool for routine diagnostic applications.
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نویسندگان مقاله | Seyed Mohammad Amin Mousavi-Rad
Applied Microbiology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran


| Shohreh Zare Karizi
Department of Biology, Faculty Member, Varamin-Pishva Branch of Azad University, Tehran, Iran


| Hamid Sedighian
Applied Microbiology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran


| Seyed Ali Mirhosseini
Applied Microbiology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran


| Hadi Esmaeili Gouvarchin ghaleh
Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran


| Jafar Amani
Applied Microbiology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/5156
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