این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Veterinary Research Forum، جلد ۱۶، شماره ۶، صفحات ۳۲۵-۳۳۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Molecular detection of the Escherichia coli heme-utilization gene A virulence factor in E. coli isolated from the feces of horses in Sumbawa island, Indonesia
چکیده انگلیسی مقاله The transmission of Escherichia coli (E. coli) containing virulent genes from animals to humans and the environment poses significant public health challenges. This study aimed to detect the virulence factor of the E. coli heme-utilization gene A (chuA) in E. coli isolated from the feces of apparently healthy horses in the island of Sumbawa, Indonesia. The study utilized 52 fecal samples from a total horse population of 283, calculated using the disease detection formula. Fresh feces were collected immediately after excretion and placed in buffered peptone water for subsequent analysis. The samples were then isolated on eosin methylene blue media and identified using biochemical tests. Identified E. coli strains were further examined for detecting the chuA gene using polymerase chain reaction techniques. The E. coli was successfully isolated and identified in 11 (21.15%) of the 52 collected fecal samples. Polymerase chain reaction analysis detected the chuA gene in 8 (15.38%) E. coli isolates at 279 bp on gel electrophoresis. The close interaction between horses and humans in the island of Sumbawa,  Indonesia, may facilitate the spread of E. coli. Thus, surveillance is needed to employ a One Health approach to monitor E. coli strains encoding the chuA gene and other virulence factors to control their dissemination.
کلیدواژه‌های انگلیسی مقاله chuA,Escherichia coli,Horse,Indonesia,Virulence Factors
نویسندگان مقاله Kholik Kholik |
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Mataram, Indonesia

Akhmad Sukri |
Department of Biology Education, Faculty of Science and Engineering, Universitas Pendidikan Mandalika, Indonesia

Katty Hendriana Priscilia Riwu |
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Mataram, Indonesia

Ieke Wulan Ayu |
Department of Argotechnology, Faculty of Agriculture, Universitas Samawa, Sumbawa, Indonesia

Ika Nurani Dewi |
Department of Biology Education, Faculty of Science and Engineering, Universitas Pendidikan Mandalika, Indonesia

نشانی اینترنتی https://vrf.iranjournals.ir/article_724068_8e88631a1a9f1aa506405794af57b033.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات