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Veterinary Research Forum، جلد ۱۶، شماره ۶، صفحات ۳۴۵-۳۵۲
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عنوان انگلیسی Conventional and molecular identification of Iranian Clostridia species associated with animal infection
چکیده انگلیسی مقاله Clostridial disease causes severe economic losses in livestock by rapidly killing ruminants. Therefore, implementing effective control approaches to prevent this fatal disease is importance. The causative agent of this disease is Clostridium spp. Accurate identification of this microorganism is crucial for effectively managing clostridial diseases in farm. There are conventional methods for detecting the disease, including microbiological and biochemical tests, many of these tests are time-consuming and exhibit low sensitivity. So, this study aims to use conventional and molecular approaches to identify Iranian isolates associated with animal infections. To achieve this, 61 samples were collected from1984 to 2024 and cultured on liver media, and subsequently subjected to microbiological and biochemical tests. For molecular identification, the DNA of isolates was extracted, and the isolates were confirmed by polymerase chain reaction (PCR) using specific primers. The results of the conventional analysis revealed that all Iranian isolates were identified as C. perfringens and its type determined by PCR assay. According to our findings, C. perfringens type A is the most prevalent strain in Iran, which predominantly found in ostriches and bird samples, followed by type D. This study underscores the presence of C. perfringens types across variety hosts and geographic locations in Iran. In conclusion, the combining conventional methods with PCR helps reliably detecting Clostridium spp. This information holds the potential to significantly contribute to the development of preventive strategies against clostridial diseases in Iran.
کلیدواژه‌های انگلیسی مقاله Biochemical tests,Clostridium perfringens,Iranian isolate,PCR
نویسندگان مقاله Lida Abdolmohammadi Khiav |
Department of Anaerobic Vaccine Research and Production, Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

Anahita Emadi |
Department of Bacterial vaccines Quality Control, Razi vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

Maryam Dadar |
Razi vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

نشانی اینترنتی https://vrf.iranjournals.ir/article_724065_db22b43a0d3bb36c5af5c6a5bfed0a2e.pdf
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