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Iranian Journal of Biotechnology، جلد ۲۳، شماره ۲، صفحات ۹۰-۱۰۳
عنوان فارسی
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عنوان انگلیسی Biotechnological Elucidation of Xinyin Tablet’s Mechanism: SIRT1 Activation Attenuates Cardiac Fibrosis Via Suppressing Endothelial-to-Mesenchymal Transition
چکیده انگلیسی مقاله Background: Xinyin tablet (XYT), a traditional Chinese medicine consisting of Ginseng, Ophiopogon, Astragalus, Ilex pubescens  Motherwort, and other medicines, is clinically used to manage chronic heart failure (HF), yet its molecular mechanisms remain underexplored.
Objectives: This study integrates biotechnological approaches to investigate how XYT mitigates cardiac fibrosis by targeting the SIRT1-mediated TGF-β/Smad signaling pathway.
Materials and Methods: Transverse aortic constriction (TAC)-induced HF mice and TGF-β1-stimulated myocardial microvascular endothelial cells (MMECs) were employed. Echocardiography, histopathology, and molecular assays (qRT- PCR, Western blotting, siRNA transfection) were utilized to assess cardiac function, fibrosis, and signaling pathways.
Results: XYT treatment significantly improved cardiac function (↑LVEF, LVFS; ↓LVIDs, LVIDd) and reduced collagen I/III deposition in TAC mice. Mechanistically, XYT upregulated SIRT1 expression while suppressing EndMT markers (↓α-SMA, ↑VE-cadherin) and TGF-β/Smad signaling (↓TGF-βR1, p-Smad2/3). Crucially, SIRT1 knockdown in MMECs abolished XYT’s inhibitory effects on EndMT and TGF-β/Smad activation, confirming SIRT1’s pivotal role.
Conclusions: These findings highlight XYT’s biotechnological relevance by linking SIRT1 activation to EndMT inhibition, offering a novel therapeutic strategy for cardiac fibrosis. This study underscores the potential of integrating
کلیدواژه‌های انگلیسی مقاله Xinyin tablet,cardiac fibrosis,Endothelial-to-mesenchymal transition,sirt1,TGF-β/Smad signaling,Molecular biotechnology
نویسندگان مقاله Qiao Li |
The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

Taochun Ye |
Department of Cardiology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Clinical Research Academy of Chinese Medicine, Guangzhou, 510405, China

Qingmin Chu |
Department of Cardiology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Clinical Research Academy of Chinese Medicine, Guangzhou, 510405, China

Xin Shang |
Department of Endocrinology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Clinical Research Academy of Chinese Medicine, Guangzhou, 510405, China

Min Liu |
Department of Endocrinology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Clinical Research Academy of Chinese Medicine, Guangzhou, 510405, China

نشانی اینترنتی https://www.ijbiotech.com/article_221079_a3330bd4a3d8cec7e15f7100266cb9df.pdf
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