Introduction: Genotoxicity is one of the safety concerns that can lead to damage at the genetic level. The observation of DLBS1033 using AMES and micronucleus tests was conducted to ensure the safety precautions.
Methods: Ames test was done using 3 strains of Salmonella typhimurium (TA98, TA100, and TA1535) with or without metabolic activation (S9 enzyme). In vitro micronucleus test was performed with prolonged exposure without S9. The variation of DLBS1033 concentrations were 100, 600, and 1200 µg/ml.
Results: The results of the Ames test (with/without S9) and in vitro micronucleus (prolonged exposure without S9) showed that DLBS1033 did not have a mutagenic effect.
Conclusion: DLBS1033 was safe and did not show any genotoxic activity.