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Iranian Journal of Biotechnology، جلد ۲۳، شماره ۳، صفحات ۶۰-۷۱
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عنوان انگلیسی Biotechnological Modulation of Gastrointestinal Function via Abdominal Massage: A Transcriptomic Analysis in a Functional Dyspepsia Rat Model
چکیده انگلیسی مقاله Background: Functional dyspepsia (FD) is a common gastrointestinal disorder with multifactorial etiology involving impaired motility, mucosal barrier disruption, and inflammation. Non-pharmacological interventions such as abdominal massage have demonstrated therapeutic benefits; however, the underlying molecular mechanisms remain largely unexplored.

Objective: To investigate the transcriptomic and molecular changes induced by abdominal massage in a rat model of FD and explore its potential as a biotechnological intervention for gastrointestinal dysfunction.

Methods: Thirty-six male Wistar rats were randomly assigned to control, FD model, and abdominal massage intervention groups. FD was induced using a multifactorial approach, and massage was administered daily for one week. Gastrointestinal motility was assessed by measuring gastric emptying and intestinal propulsion rates. Duodenal tissues were analyzed using transcriptome sequencing, differential gene expression profiling, Gene Ontology (GO) annotation, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Serum levels of TNF-α, IFN-γ, and IL-1β, as well as expression of tight junction proteins Occludin and ZO-1, were assessed via ELISA, immunofluorescence, and Western blotting.

Results: FD rats exhibited reduced motility and villus atrophy, with elevated inflammatory cytokines and downregulated tight junction proteins. Transcriptomic profiling revealed significant gene expression changes in pathways related to digestion, nutrient absorption, immune modulation, and epithelial barrier integrity. KEGG analysis highlighted the involvement of PI3K-Akt signaling, neuroactive ligand-receptor interaction, and absorption-related pathways. Abdominal massage reversed these alterations, improving motility, reducing inflammation, and restoring mucosal integrity.

Conclusion: This study demonstrates that abdominal massage exerts biotechnologically relevant effects on gastrointestinal function in FD through transcriptomic regulation of immune and barrier-related pathways. These findings support its potential as a non-invasive therapeutic approach integrating traditional techniques with modern molecular biotechnology.
کلیدواژه‌های انگلیسی مقاله Functional dyspepsia,Abdominal Massage,Transcriptomics,PI3K-Akt signaling,Intestinal Barrier
نویسندگان مقاله Yonggang Yang |
Acupuncture and moxibustion Massage College of Changchun University of Chinese Medicine, Changchun 130117, China

Xurong Liang |
Acupuncture and moxibustion Massage College of Changchun University of Chinese Medicine, Changchun, China

Jiyu Yang |
Changchun Humanities and Sciences College, Changchun, China

Jiyu Yang |
Changchun Humanities and Sciences College, Changchun, China

Xin Wang |
Acupuncture and moxibustion Massage College of Changchun University of Chinese Medicine, Changchun, China

نشانی اینترنتی https://www.ijbiotech.com/article_224223_2f4aace3f065889114d28ef991e5fb77.pdf
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