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Iranian Journal of Biotechnology، جلد ۲۳، شماره ۳، صفحات ۱۰۹-۱۱۳
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عنوان انگلیسی Impact of a Synthetic Androgen on Milt Quality and Semen Preservation of Gilthead Seabream, Sparus aurata (Carl Linnaeus, 1758)
چکیده انگلیسی مقاله Background: The use of synthetic hormones, particularly androgens, for induced breeding of fish has been a long-standing trend to improve the milt quality of male fish.

Objectives: To evaluate the impact of Testosterone propionate (TP) on the milt quality and short-term preservation of Gilthead Seabream semen at 4oC in oxygen and Fish Ringer’s medium.

Materials and Methods: Mature male fishes were given intra-muscular injection of TP at a dose of 1.0 ml/ Kg. After 36 hours, milt was assessed for sperm motility, density, sperm viability and colour. Semen preserved at 4 oC in oxygen and Fish Ringer’s medium was examined for sperm motility (%), motility duration (sec) and viability (%) at 24,48,72,96 and 120 h.

Results: Significant change in colour or pH of the milt was not observed. Milt’s consistency changed from being thick to more watery. The hormone-injected fish had an increased level of milt volume and sperm density than the control. Milt stored in an oxygen atmosphere showed higher sperm cell quality than that stored in Fish Ringer’s medium.

Conclusion: TP injection at 1.0 ml/ Kg enhances sperm concentration, motility and viability of Gilthead Seabream, S.aurata.
کلیدواژه‌های انگلیسی مقاله Keywords Milt quality,Preservation,Seabream,Testosterone Propionate
نویسندگان مقاله Sambhu Chithambaran |
King Abdulaziz Univerity

Mamadouh Ali Al Harbi |
Faculty of Marine Sciences, King Abdulaziz University

نشانی اینترنتی https://www.ijbiotech.com/article_224225_0a62a366fe74fe7d7639ac51c3ac2b2a.pdf
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