این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Research in Pharmaceutical Sciences، جلد ۲۰، شماره ۳، صفحات ۳۴۳-۳۵۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Klotho microinjection into the RVLM attenuates acute kidney injury via interaction with the cholinergic anti-inflammatory pathway in rats
چکیده انگلیسی مقاله Background and purpose: The Klotho ( Klo ) gene, an aging suppressor in rats, accelerates aging when disrupted and extends lifespan when overexpressed. It encodes a transmembrane protein primarily expressed in renal tubules. This study investigated the protective effects of central Klo, both alone and in combination with cholinergic anti-inflammatory pathway (CAP) inhibition, against ischemia-reperfusion injury (IRI)-induced acute kidney injury. The current study evaluated the expression of inflammatory and anti-inflammatory genes (including Il1b, Tnfa, Tgfb, Trem2, and Il10 ) in the kidney, alongside plasma levels of creatinine (Cr), blood urea nitrogen (BUN), and signs of acute tubular injury. Experimental approach: Klo was microinjected into the rostral ventrolateral medulla, and CAP inhibition was achieved through intraperitoneal administration of mecamylamine (Mec). Real-time RT-PCR and hematoxylin and eosin staining were used for gene expression analysis and histopathological examination, respectively. Findings/Results: The results showed elevated Cr and BUN levels, tubular injury, and increased inflammatory gene expression in IRI and IRI + Mec groups, as well as reduced Il10 in the IRI + Mec group. Klo exhibited protective effects. Elevated Tgfb expression was seen in IRI + Klo and IRI + Mec + Klo groups one week post-surgery. Conclusion and implications: These findings indicated Klo potential to extend lifespan and protect against age-related diseases, including kidney disease and inflammation, via neural modulation of peripheral immunity.    
کلیدواژه‌های انگلیسی مقاله Acute kidney injury,Cholinergic anti-inflammatory pathway,Klotho,Mecamylamine,Rostral ventrolateral medulla.
نویسندگان مقاله | Fatemeh Ahmadi
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Elahe Amohashemi
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Mohammad Kazemi
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Hossein Salehi
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Parham Reisi


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/2311
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات