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Iranian Biomedical Journal، جلد ۲۹، شماره ۳، صفحات ۵-۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Development of Experimental Platforms to Assess Helicobacter pylori HopQ Interaction with Host CEACAM Molecules
چکیده انگلیسی مقاله
Background: Helicobacter pylori is an extracellular bacterium responsible for various gastrointestinal diseases, such as peptic ulcers and gastric cancer. It uses multiple mechanisms to colonize the harsh, acidic environment of the stomach and establish its pathogenic processes, mostly through CagA translocation. While cell surface integrin molecules were previously believed to be the main mediators anchoring H. pylori and facilitating this process, recent studies highlight the critical role of the interaction between the bacterial adhesin HopQ and host CEACAMs in CagA translocation and subsequent pathogenic signaling.
Methods: Recombinant proteins including HopQ, HopQ-GFP, HopQ-HRP, and C1ND were produced via gene cloning, expression, and purification techniques. Ligand-receptor interactions were evaluated using FACS analysis along with antigen- and cell-based ELISA assays.
Results: In this study, we have developed antigen and cell-based platforms using recombinant fusion proteins (HopQ-GFP and HopQ-HRP) that effectively interact with recombinant C1ND, as well as various CEACAM molecules expressed on gastric cell lines (MKN45 and AGS).
 Conclusion: These assay platforms enable detailed investigation of the HopQ-CEACAM interaction and support high-throughput screening of inhibitors, facilitating the identification of potential drugs or vaccine candidates targeting H. pylori infection.
کلیدواژه‌های انگلیسی مقاله Adhesion, Enzyme-linked immunosorbent assay, Ligands, Receptor
نویسندگان مقاله | Nazanin Shans
Biotechnology Dept


| Maryam Esmaeili
Biotechnology Dept


| Kimia Abraheh
Biotechnology Dept


| Niloofar Asadi Hanjani
Biotechnology Dept


| Maedeh Farrokhi
Biotechnology Dept


| Negar Sardarpour
Biotechnology Dept


| Yeganeh Talebkhan
Biotechnology Dept


| Fatemeh Kazemi-Lomedasht
Biotechnology Dept


| Esmat Mirabzadeh
Biotechnology Dept


| Marjan Mohammadi
Biotechnology Dept
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-670-10&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Medical Biotechnology
نوع مقاله منتشر شده مقاله کامل
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