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Iranian Journal of Public Health، جلد ۵۴، شماره ۸، صفحات ۱۷۷۶-۱۷۸۵
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عنوان انگلیسی Silver Nanoparticles Synthesised Using Sargassum Angustifolium Can Effect on MiR-25 and MiR-143 Expression in Acute Lymphoblastic Leukemia
چکیده انگلیسی مقاله Background: One of the treatments of acute lymphoblastic leukemia (ALL) is chemotherapy but it can destroy other normal cells as well as cancer cells, which can lead to infection and bleeding. The widespread consumption of nanoparticles synthesized biogenically by seaweed is because of their easy accessibility and usefulness. We aimed to investigate silver nanoparticles synthesised using Sargassum on the expression of miR-25 and 143 in Jurkat cell line. Methods: In an interventional study in 2019, Bushehr, Iran, following culture of the Jurkat cell line with 95% survival rate, we applied different drug concentrations to 20,000 cell lines and control cells separately to determine maximum cell growth inhibition and IC50. After treatment for 48 h, RNA extraction was performed, and Real Time PCR was used to evaluate miR-25 and 143 expressions. Results: MiR-25 expression in the groups that treated with the maximum dose and IC50 of silver nanoparticles and algae extract was decline but did not differ significantly from that of the control group. Conversely, miR-143 showed a remarkable decline in both treatment groups (P<0.0001). After treating PBMC with nanoparticles the expression level of miR-25 and miR-143were not significantly different. Conclusion: The level of miR-25 and 143 expressions were decrease in the IC50 dosage of silver nanoparticles and algae extract produced. The level of miR-25 expression decreased more in the treated Jurkat cell line than the level of miR-143 expression in normal lymphocytes. Given the oncogenicity of miR-25 in the Jurkat cell line, it is suggested that decreasing level of this microRNA can help to apoptosis of leukemic cells.
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نویسندگان مقاله | Sepideh Valipour
Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran


| Narges Obeidi
1. Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran 2. The Persian Gulf Marine Biotechnology Research Center, Bushehr, Iran


| Gholamreza Khamisipour
Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran


| Seyed Amin Mohammadi
Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran


| Maryam Ghaemi
Iranian National Institute for Oceanography and Atmospheric Science, Bushehr, Iran


| Farzad Farhangdoost
Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran


| Hamideh Malekhayaty
Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran


| Maedeh Darzipour
Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
نشانی اینترنتی https://ijph.tums.ac.ir/index.php/ijph/article/view/34855
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