این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Pathology، جلد ۲۰، شماره ۴، صفحات ۴۰۴-۴۱۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Interobserver Variability in Ki-67 Index Assessment of Gastroenteropancreatic Neuroendocrine Neoplasms: A Comparative Evaluation of Three Counting Methods
چکیده انگلیسی مقاله Background & Objective: Accurate evaluation of the Ki-67 proliferation index is critical in the grading and prognostication of neuroendocrine neoplasms (NENs). This study aimed to compare three different methods of Ki-67 index assessment in primary gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs).
Methods: The Ki-67 proliferation index was assessed by immunohistochemical (IHC) staining in 41 cases of primary GEP-NENs. Two pathologists independently evaluated the Ki-67 index using two visual estimation methods: eyeballing and eye-counting under a light microscope. A third method involved manual counting of Ki-67–positive and –negative nuclei on a captured image using ImageJ software. Interobserver and intermethod reproducibility were analyzed using Pearson’s correlation coefficient (R) and the intraclass correlation coefficient (ICC) to assess agreement among the four observers and between each observer and the manual method.
Results: The ICC among the four observers was 0.993, indicating excellent interobserver agreement. The ICC between each observer’s score and the manual counting method was 0.994, also demonstrating high concordance.
Conclusion: All three methods, eyeballing, eye-counting, and manual counting using ImageJ, proved to be comparably effective in assessing the Ki-67 proliferation index in GEP-NENs. Notably, the simpler microscopic techniques of eyeballing and eye-counting showed excellent agreement with the manual image-based method, supporting their reliability in routine practice.
کلیدواژه‌های انگلیسی مقاله Neuroendocrine neoplasms,Gastroenteropancreatic neuroendocrine tumors,Ki-67,MIB-1,Visual estimation,ImageJ
نویسندگان مقاله Emerine Selma Edwin |
Department of Pathology, Kasturba Medical College Mangalore, Manipal Academy of Higher education, Manipal, India

Pooja K Suresh |
Department of Pathology, Kasturba Medical College Mangalore, Manipal Academy of Higher education, Manipal, India

Hema Kini |
Department of Pathology, Kasturba Medical College Mangalore, Manipal Academy of Higher education, Manipal, India

Saraswathy Sreeram |
Department of Pathology, Kasturba Medical College Mangalore, Manipal Academy of Higher education, Manipal, India

Jyoti Kini |
Department of Pathology, Kasturba Medical College Mangalore, Manipal Academy of Higher education, Manipal, India

Sridevi Basavaiah |
Department of Pathology, Kasturba Medical College Mangalore, Manipal Academy of Higher education, Manipal, India

Sneha Rao |
Department of Pathology, Kasturba Medical College Mangalore, Manipal Academy of Higher education, Manipal, India

نشانی اینترنتی https://ijp.iranpath.org/article_728540_cc8ef7b2ba8e69d616faf3eccecda69f.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات