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Iranian Journal of Veterinary Science and Technology، جلد ۱۷، شماره ۳، صفحات ۱-۷
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عنوان انگلیسی Biochemical and Molecular Characterization of Pseudomonas putida, P. fluorescens and P. aeruginosa Isolate from Oreochromis niloticus (TILAPIA ) and Clarias gariepinus (AFRICAN CATFISH)
چکیده انگلیسی مقاله Bacterial pathogens are a major cause of economic losses in aquaculture and pose serious threat to public health. This study investigates the phenotypic and the genomic characteristics of pathogenic Pseudomonas species in two finfish species, Oreochromis niloticus (Tilapia) and Clarias gariepinus (African catfish), sourced from the Galadima fish market in Kano metropolis, Nigeria. Twenty fish samples, including 10 Tilapia and 10 African catfish were randomly selected and tissue samples (liver, spleen, intestine and gills) were collected for analysis. Pseudomonas spp. were screened via culturing and isolation techniques and biochemical tests. Molecular identification was carried out based on 16S rRNA gene sequence analysis. Out of all the samples analyzed,  6 % tested positive for Pseudomonas spp., including P. putida (5 %), P. aeruginosa (1.25 %), and P. fluorescens (1.25 %). This study confirms the presence of potentially pathogenic Pseudomonas species in commercially important finfish species from the Galadima fish market in Kano, Nigeria. These findings highlight the need for regular monitoring and molecular surveillance of bacterial pathogens in aquaculture products to prevent economic losses and safeguard public health.
کلیدواژه‌های انگلیسی مقاله Tilapia,African catfish,Sequencing,Bacteria
نویسندگان مقاله Mannir Dahiru Usman |
Department of Veterinary Medicine, Bayero University Kano, Nigeria.

Clarance Suh YAH |
Faculty of Health Science Research Office (HSSO). Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

Victoria Folakemi Akinjogunla |
Department of Fisheries and Aquaculture, Bayero University Kano, Nigeria.

Tuaheed Abubakar Muazu |
Department of Veterinary Anatomy, Bayero University Kano, Nigeria.

Abdulrazak Lawal |
Department of Veterinary Public health and Preventive Medicine, Bayero University Kano, Nigeria.

Yusuf Abubakar |
Department of Veterinary Public health and Preventive Medicine, Federal University of Agriculture Zuru, Nigeria.

Khalid Shuaibu HASSAN |
Centre for Dryland Agriculture, Bayero University Kano, Nigeria.

Jamiu Olatoye Salako |
Department of Fisheries and Aquaculture, Bayero University Kano, Nigeria.

Christian Anuoluwapo OLASENI |
Department of Fisheries and Aquaculture, Bayero University Kano, Nigeria.

نشانی اینترنتی https://ijvst.um.ac.ir/article_46816_7950b94a402e94e683c7e91fcdcef728.pdf
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