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Nanomedicine Journal، جلد ۱۲، شماره ۴، صفحات ۶۳۷-۶۴۷
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عنوان انگلیسی Nanozyme-based Aptasensor for colorimetric detection of vitamin D3 in milk
چکیده انگلیسی مقاله Objective(s): The growing demand for food production necessitates the development of advanced detection methods to ensure food quality and prevent adulteration. This study presents a colorimetric aptasensor specifically designed to detect vitamin D3 in milk, utilizing the unique properties of nanozymes in combination with aptamers.Materials and Methods: The method utilizes the peroxidase-like activity of bare iron oxide magnetic nanoparticles, which interact electrostatically with aptamers, leading to a color change in the TMB-H2O2 solution through a Fenton-like redox reaction.Results: The results demonstrated that both the choice of buffer and the concentration of vitamin D3 significantly impact the catalytic activity of the nanozymes. In addition, using iron oxide nanozymes offers several advantages, including enhanced stability, straightforward interactions, tunable activity, and effective background color removal through magnetic separation.Conclusions: This innovative approach enhances the reliability of vitamin D3 detection in dairy products and holds broader implications for food safety and quality assurance. The findings highlight the potential of integrating nanotechnology and biosensing techniques to address critical challenges in food monitoring and safety. By tackling these issues, this research contributes to the development of effective strategies for ensuring food integrity and safeguarding consumer health in an era where food adulteration continues to be a pressing concern.
کلیدواژه‌های انگلیسی مقاله Iron oxide nanoparticles, Vitamin D3, Peroxidase, Oligonucleotides, colorimetry, Milk
نویسندگان مقاله | Abolghasem Rahmani
Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Adele Rafati
Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran|Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran


| Zahra Valipanah
Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Pooria Gill
Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran|The Health of Plant and Livestock Products Research Center, Mazandaran University of Medical Sciences, Sari, Iran
نشانی اینترنتی https://nmj.mums.ac.ir/article_26340.html
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