این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Ecopersia، جلد ۸، شماره ۲، صفحات ۱۲۵-۱۳۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Biochemical Characterization of Lysozyme Extracted from Common Carp, Cyprinus carpio
چکیده انگلیسی مقاله Aim: The aim of the present study was to investigate the biochemical characterizations of the lysozyme enzyme for evaluation of its importance in the immune system of the common carp, Cyprinus carpio.
Materials & Methods: In the present study, lysozyme was extracted from the spleen of common carp, Cyprinus carpio. Then, partially purified by ammonium sulfate and some properties such as optimum pH and temperature as well as the effects of different salt concentrations of NaCl, MgCl2, KCl, and urea on enzyme activity were evaluated. The enzyme activity was assayed using a suspension of Micrococcus lysodeikticus as a substrate.
Findings: The optimum pH and temperature were found 4 and 50°C, respectively. Furthermore, lysozyme activity was found to be dependent on salt concentration.
Conclusion: Based on the results, it's been concluded that lysozyme extracted from the spleen of the C. carpio has its optimum activity at high temperature and low pH condition and its activity could be continued with the presence of different salt compounds which all these are related to the environmental conditions of natural habitats of the C. carpio and showed that lysozyme could be one of the key factors of the immune system in this species.
کلیدواژه‌های انگلیسی مقاله common carp,Lysozyme,Enzymology,immune system
نویسندگان مقاله M. Ghalambor |
Biology Department, Science Faculty, Dezful Branch, Islamic Azad University, Dezful, Iran

Z. Eslamifar |
Biochemistry Department, Medical Faculty, Dezful University of Medical Sciences, Dezful, Iran

Z. Khoshnood |
Biology Department, Science Faculty, Dezful Branch, Islamic Azad University, Dezful, Iran

نشانی اینترنتی https://ecopersia.modares.ac.ir/article_17288_67d76cb7a3472cc97bb7dc903e18de4a.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات