این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Agricultural Science and Technology، جلد ۲۶، شماره ۲، صفحات ۴۱۵-۴۲۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Identification of Noctuidae and Nolidae (Lepidoptera) Major Crop Pest Species in Iran: A Combination of Morphological and DNA Barcoding Approaches
چکیده انگلیسی مقاله Larvae of numerous Noctuidae and Nolidae species have significant annual economic losses in agriculture. DNA-based diagnostics have been proposed as an effective way to accelerate the identification and discovery of new species. This study aimed to determine the utility of up to 642 bp Cytochrome c Oxidase subunit I (COI) barcodes for identifying 12 major Iranian Noctuidae and Nolidae crop pests and confirming morphological identifications based on classical taxonomy. We combined molecular and morphological analysis to identify 53 specimens collected from populations throughout Iran. The results indicated the presence of a distinct barcode gap for different pest species. The mean interspecific sequence divergence (Kimura 2-parameter) was an order of magnitude (10.0%) greater than the mean intraspecific sequence divergence (0.29%). This combination of DNA and morphological analyses identified 13 species, one of which was previously unknown and may represent a new previously overlooked Earias species. There were no, or very few, sequences from Iran in international databases for some of the test species. Here, we increase the number of specimens from Iran and aid in taxonomic interpretation. The current study will aid in the identification of the most common Noctuidae and Nolidae major pest species in Iran, regardless of the observer’s taxonomic skills, developmental stage of the vouchers, as well as sex, or insect preservation condition. Our data enables researchers and practitioners involved in the bio-surveillance of insect pests to identify taxa based on simple DNA sequence comparisons quickly. DNA barcoding in conjunction with morphological identifications can provide secondary evidence supporting morphological identifications and improve taxonomic resolution.
کلیدواژه‌های انگلیسی مقاله Barcoding gap,Genetic distance,Molecular Identification,Nucleotide divergence
نویسندگان مقاله S. Shahreyari-Nejad |
Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Islamic Republic of Iran.

M. Esfandiari |
Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Islamic Republic of Iran.

A. Rasekh |
Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Islamic Republic of Iran.

M. S. Mossadegh |
Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Islamic Republic of Iran.

A. Shirvani |
Department of Plant Protection, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Islamic Republic of Iran.

نشانی اینترنتی https://jast.modares.ac.ir/article_16930_be66f22368ab780c92156a7f785d295a.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات