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Journal of Agricultural Science and Technology، جلد ۲۵، شماره ۳، صفحات ۵۸۱-۵۹۴
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عنوان انگلیسی Comparative Analysis of Differential Exon Usage between the Breeds of Sheep and Goats Using RNA-Seq Data
چکیده انگلیسی مقاله Alternative splicing, alternative transcript start site, and alternative transcript polyadenylation site are the main factors resulting in diversity of the transcripts of a gene. The main objectives of this study were to analyze the alternation process in breeds of sheep and goat, and to identify its role in differentiation of breeds of a species. RNA-seq data were prepared from ovarian tissue of two breeds of Shal and Sangsari sheep and two breeds of Tibetan and Jintang black goats. Reads were aligned to the reference genome and significant genes with respect to differential exon usage were identified. The statistical comparison revealed that 8,104 genes were significantly different in exon usage between the sheep breeds and 173 genes differed between the goat breeds. Out of the 121,861 studied exons, only 22.7% were preserved during future generations between the breeds, of which 99.3% did not display any alternatives. The high protection was probably due to the lack of involvement of the exons in alternative process. The genes with differential exon usage in goat had a higher percentage of alternatives than those in sheep. The interracial analysis showed that alternative splicing was the most influential type of alternatives in the breeds of sheep and goats. It seems that the conservation process of the exons is related to the contribution of these exons in alterative process in both sheep and goat breeds. The significant PI3K-Akt and alternative splicing pathways play a role in cell growth, development of ovaries, and mRNAs splicing.
کلیدواژه‌های انگلیسی مقاله Alternative splicing,Interethnic analysis,Ovarian tissue,Transcription
نویسندگان مقاله F. Nikookalam Azim |
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

A. A. Masoudi |
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

A. Ehsani |
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares UniversityTehran, Iran

R. Vaez Torshizi |
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

P. Shariati Gazgazareh |
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

نشانی اینترنتی https://jast.modares.ac.ir/article_16760_8e370aa20aec9f2177415df3fb54937b.pdf
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