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Journal of Agricultural Science and Technology، جلد ۲۰، شماره ۴، صفحات ۷۳۳-۷۴۴
عنوان فارسی استفاده از روش Multiplex PCR برای تشخیص همزمان دو گونه غالب از کنه‌های تارتن جنس تترانیخوس (Acari: Tetranychidae) در گلخانه‌های ایران
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عنوان انگلیسی A Multiplex PCR Assay for Simultaneous Discrimination of Two Predominant Spider Mites of the Genus Tetranychus (Acari: Tetranychidae) in Greenhouses of Iran
چکیده انگلیسی مقاله Molecular species identification has become more ubiquitous in diagnostics of insects, particularly in situations where morphological identification is a laborious or time-consuming process. Tetranychid mites are serious agricultural pests. Identification of tetranychid mites is usually arduous and requires a high level of taxonomic expertise because of their minute size, their close morphological similarities as well as limited number of diagnostic characters. Most species of the spider mites of the genus Tetranychus Dufour in Iran are morphologically similar, differing only in the diameter of the aedeagal knob in males. Because this genus contains many important pests, the unambiguous identification of species is crucial for effective pest management. In this study, a single-step multiplex Polymerase Chain Reaction (multiplex PCR) was used to discriminate two predominant spider mite species occurring in greenhouses in Iran: Tetranychus urticae Koch and Tetranychus turkestani (Ugarov and Nikolskii). The single-step multiplex PCR developed here, based on ITS regions, is rapid, reliable, sensitive and relatively simple. The entire identification protocol from DNA extraction to electrophoresis could be completed in four hours. Moreover, it is adequately simple to be implemented in any molecular laboratory.
کلیدواژه‌های انگلیسی مقاله DNA-based Identification,Molecular acarology,Tetranychid mites,Tetranychus turkestani,Tetranychus urticae
نویسندگان مقاله S. Sinaie |
Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box: 91779-48974, Islamic Republic of Iran.

H. Sadeghi Namaghi |
Academic member of Ferdowsi University of Mashhad

L. Fekrat |
Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box: 91779-48974, Islamic Republic of Iran.

نشانی اینترنتی https://jast.modares.ac.ir/article_16179_e80834ff79f552f2048eba581faff5c7.pdf
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