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JCR 2016
جستجوی مقالات
چهارشنبه 19 آذر 1404
Journal of Crop Protection
، جلد ۸، شماره ۱، صفحات ۳۳-۴۳
عنوان فارسی
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عنوان انگلیسی
Effect of quercetin on some digestive enzyme activity via crustacean cardioactive peptide (CCAP) content of the midgut of the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)
چکیده انگلیسی مقاله
Efficacy of quercetin on
α
-amylase, lipase and protease activities via crustacean cardioactive peptide (CCAP) content of the midgut of the diamondback moth,
Plutella xylostella
(L.) was investigated. Fresh cabbage leaf discs were dipped in quercetin solution at different concentrations (100, 500 and 1000ppm) for 10 seconds. Third instar larvae of
P. xylostella
were fed on leaf dipped in quercetin solutions for 5 days. α-Amylase, lipase and protease activities were evaluated for 5 days. Quercetin significantly decreased lipase, protease and α-amylase activities in the midgut. The results of competitive ELISA showed that different concentration of quercetin had no effect on short neuropeptide F, tachykinin-4 and allatostatin content of the midgut, but it was shown that quercetin (500 and 1000ppm) decreased CCAP content of the midgut. Moreover, incubation of dissected midgut with CCAP increased α-amylase, lipase and protease activities. The injection of CCAP into the hemocoel clearly increased
α
-amylase, lipase and protease activities. Here, for the first time, it was confirmed that feeding on leaf dipped in quercetin, decreases CCAP content in the midgut of
P. xylostella
, that itself led to decrease of
α
-amylase, protease and lipase activities.
کلیدواژههای انگلیسی مقاله
Plutella xylostella,Quercetin,Crustacean cardioactive peptide,α-amylase,Protease
نویسندگان مقاله
Azam Mikani |
Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
نشانی اینترنتی
https://jcp.modares.ac.ir/article_1413_eebf2e84d3360b66a070db0876e25156.pdf
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