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International Journal of Fertility and Sterility، جلد ۱۹، شماره ۴، صفحات ۴۲۱-۴۲۸
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عنوان انگلیسی Navigating The CeRNA Axis by Bioinformatics and Experimental Validation: Identification of ADIRF-AS1, miR-191-5p, and EGR1 as Key Players in Endometrial Carcinoma Progression
چکیده انگلیسی مقاله Background: Endometrial carcinoma (EC) is a significant gynecologic malignancy. Investigating competing endogenous
RNA (ceRNA) networks, including long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger
RNAs (mRNAs), offers insights into EC's molecular intricacies and may improve therapeutic and diagnostic strategies.
This study focuses on the ceRNA axis, particularly the interactions of lncRNA-miRNA-mRNA-Gene, using
TCGA-UCEC database analysis and experimental validation.
Materials and Methods: In this case-control study, differentially expressed lncRNAs (DElncRNAs), microRNAs (DEMIs),
and genes (DEGs) were identified, highlighting ADIRF-AS1 as a potential target. Validated interactions between
ADIRF-AS1, miR-191-5p, and EGR1 were established, with significant ADIRF-AS1/EGR1 correlation. Protein-protein
interaction (PPI) analysis identified 21 proteins linked to EGR1, with gene ontology (GO) analysis revealing roles in
myeloid cell differentiation. Expression levels of genes, lncRNA, and microRNAs were evaluated by quantitative reverse
transcription polymerase chain reaction (qRT-PCR). Western blot analysis was applied for protein evaluation.
Results: RT-qPCR results showed that the RNA expression levels of ADIRF-AS1 and EGR1 genes in endometrial
cancer and hyperplasia samples were significantly lower (P<0.001) than control samples. Also, the expression level
of miR-191-5p in endometrial cancer tissues was significantly higher than patients with hyperplasia (P<0.001) and
normal samples (P<0.001). Western blot results also showed that the protein level of EGR1 in endometrial cancer
samples was significantly lower than control samples (P<0.001).
Conclusion: Here, we observed an interaction between lncRNA ADIRF-AS1and hsa-miR-191-5p, and also, ADIRFAS1
downstream effects on EGR1 in EC, that seems may be a suggesting therapeutic and diagnostic targets. Further
research could explore its clinical relevance in endometrial carcinoma.
کلیدواژه‌های انگلیسی مقاله Bioinformatics,Early Growth Response 1,Endometrial Cancer,Hyperplasia
نویسندگان مقاله Azadeh Rezaei |
Biochemistry Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Rahim Rostami |
Biochemistry Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Shahram Teimourian |
Genetic Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Saman Morovat |
Genetic Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Elmira Mehdinia |
Biochemistry Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Afsaneh Tehranian |
Department of Gynaecology and Obstetrics, Arash Women&apos;s Hospital, Tehran University of Medical Sciences, Tehran, Iran

Soudabeh Fallah |
Biochemistry Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

نشانی اینترنتی https://www.ijfs.ir/article_718047_6ca2f76bc345ef4ec7fe59023a2a2ef8.pdf
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