این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۷، شماره ۵، صفحات ۷۴۱-۷۵۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Phylogenetic analysis of blaNDM genes of carbapenem resistant uropathogens isolated from federal tertiary care hospital, Pakistan: insights into the evolution and dissemination of drug resistance
چکیده انگلیسی مقاله Background and Objectives: Global health is seriously threatened by the rise of carbapenem-resistant Enterobacterales (CRE). The blaNDM gene, a key carbapenemase coding gene, causes global health concern due to its multidrug resistance and easy spread through mobile genetic elements. This study aimed to identify and genetically characterize the blaNDM genes from uropathogens, its antibiotic susceptibility, and its correlation with global sequences. Materials and Methods: Urine samples were processed following microbiological guidelines. Isolates were identified using API-20E. Antibiotic susceptibility was tested using disc diffusion method, and bacterial DNAs were extracted for blaNDM gene sequencing for phylogenetic analysis. Results: CREs were detected in 11.92% (n=51) of the 428 Enterobacterales. Among CRE isolates, 45% (n=23) were positive for blaNDM gene harbored by Klebsiella pneumoniae (57%), followed by Escherichia coli (26%). Uropathogenic CRE, harboring blaNDM, revealed susceptibility of 34.78%, 60.87%, and 65.22% to amikacin, nitrofurantoin, and fosfomycin respectively. The blaNDM-5 variant was most common (69.57%), followed by blaNDM-1 (26.09%) and blaNDM-7 (4.35%). Phylogenetic analysis revealed that blaNDM variants exhibit diverse relationships with Pakistani and worldwide sequences. Conclusion: The significant presence of blaNDM in uropathogens, along with extensive antibiotic resistance, underscores the urgent need for continuous monitoring and antibiotic stewardship programs to manage the growing threat of CRE infections.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Muhammad Shafiq
Department of Biosciences, Comsats University, Islamabad, Pakistan


| Amnah Khan
Department of Biosciences, Comsats University, Islamabad, Pakistan


| Muhammad Hyder
Department of Biosciences, Comsats University, Islamabad, Pakistan


| Umme Farwa
Department of Pathology, Fazaia Medical College, Air University, Islamabad, Pakistan


| Farhan Kursheed
Department of Microbiology, PMAS Arid Agriculture University, Rawalpindi, Pakistan


| Sana Khan
Department of Pathology, Pakistan Institute of Medical Sciences (PIMS), Islamabad, Pakistan


| Ayesha Siddiqa
Department of Pathology, Pakistan Institute of Medical Sciences (PIMS), Islamabad, Pakistan


| Iftikhar Ahmed
National Culture Collection of Pakistan (NCCP), Land Resources Research Institute (LRRI), National Agriculture Research Centre, Park Road, Islamabad, Pakistan


| Muhammad Saeed
Department of Biosciences, Comsats University, Islamabad, Pakistan


| Muhammad Fiaz
Department of Pathology, Pakistan Institute of Medical Sciences (PIMS), Islamabad, Pakistan
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/5216
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات