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Iranian Journal of Biotechnology، جلد ۲۳، شماره ۴، صفحات ۳۰-۵۳
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عنوان انگلیسی Development of a CTL Epitope-Based Vaccine Targeting Tumor Antigens in Esophageal Cancer: An Immunoinformatics Study
چکیده انگلیسی مقاله Background:

Esophageal cancer is a highly aggressive malignancy with poor global survival rates and limited efficacy from conventional therapies. Immunotherapy has shown promise, yet its effectiveness remains limited as a standalone approach. The identification of tumor-specific antigens offers a targeted strategy for therapeutic vaccine development.

Objectives:

This study aimed to design a cytotoxic T lymphocyte (CTL)-based multi-epitope vaccine targeting immunodominant tumor-specific antigens associated with esophageal cancer using computational immunoinformatics and structural bioinformatics techniques.

Methods:

Fourteen validated CTL epitopes were selected based on immunogenicity, antigenicity (VaxiJen), IFN-γ inducibility, and structural stability. These epitopes were linked using AAY linkers to enhance processing by MHC class I molecules. The vaccine construct was evaluated for physicochemical properties, allergenicity, and toxicity. Structural modeling was performed using Robetta, and the model was validated by ProSA, ERRAT, and Ramachandran plot analysis. Molecular docking with TLR4 and MHC-I alleles, molecular dynamics simulation (iMODS), and MM/GBSA free energy calculations were used to assess binding affinity and structural stability. Immune simulations and population coverage analyses were also performed.

Results:

The designed vaccine showed strong antigenicity (0.6199), was non-toxic and non-allergenic, and exhibited excellent stability and solubility. Molecular docking revealed strong binding with TLR4 (−1125.3 kcal/mol), and MM/GBSA analysis confirmed favorable binding free energy (−93.41 kcal/mol). Immune simulations indicated robust T-cell responses. Population coverage was highest in Oceania (64.14%) and East Asia (60.66%). Codon optimization and in silico cloning confirmed high expression potential in E. coli.

Conclusion:

The vaccine demonstrates strong potential as a safe, stable, and broadly applicable immunotherapeutic candidate against esophageal cancer, pending experimental validation.
کلیدواژه‌های انگلیسی مقاله Esophageal Cancer,CTL-based vaccine,Immunoinformatics,Reverse Vaccinology,Structural bioinformatics
نویسندگان مقاله Hongyou Cao |
Department of Oncology，Ankang People's Hospital，Ankang 725000，China

Fengjiao Ding |
Department of Laboratory Medicine，Ankang Central Hospital, Ankang 725000，China

نشانی اینترنتی https://www.ijbiotech.com/article_229902_757182d0816d835a214c7a44148bdd4f.pdf
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