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Iranian Journal of Biotechnology، جلد ۲۳، شماره ۴، صفحات ۱۱۵-۱۲۶
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عنوان انگلیسی Mathematical Modeling and Thermodynamic Integration for Precision PCR Optimization
چکیده انگلیسی مقاله Background: The optimization of Polymerase Chain Reaction (PCR) conditions plays an instrumental role in improving DNA amplification sensitivity and specificity. These enhancements are critical for molecular diagnostics, forensic science, and genetic research applications. Among the underlying factors, MgCl₂ concentration and Tm are paramount in the performance of PCR. The determination of these two parameters requires the combination of sound theoretical and empirical approaches for optimization.

Objectives: This study aims to develop a predictive modeling framework for precisely optimizing MgCl₂ concentration and Tm in PCR conditions.

Materials and Methods: The study developed predictive models using multivariate Taylor series expansion and thermodynamic functions integrated with 120 PCR primers across various species. Ridge, Lasso, and Elastic Net multiple regression analyses were applied to fine-tune these models.

Results: The models demonstrated excellent predictive capabilities, achieving an R² =0.9942 for MgCl₂ concentration and 0.9600 for Tm. These findings underscore the efficacy of the proposed optimization strategy.

Conclusion: The research provides a tight framework for optimizing PCR conditions and enhancing specificity and sensitivity in DNA amplification. More importantly, it is based on an approach that combines theoretical modeling with empirical validation, thus offering valuable insight into the advancement of PCR-based methodologies.
کلیدواژه‌های انگلیسی مقاله Polymerase Chain Reaction,MgCl₂,Thermodynamics,optimization,Melting Temperature (Tm),Taylor Series Expansion
نویسندگان مقاله Hadja Fatima Tbahriti |
Higher School of Biological Sciences of Oran, Oran, Algeria

Aicha Zerrouki |
Higher School of Biological Sciences of Oran, Oran, Algeria

Fatima Zohra Mahammi |
Higher School of Biological Sciences of Oran, Oran, Algeria

Ali Boukadoum |
Higher School of Biological Sciences of Oran, Oran, Algeria

نشانی اینترنتی https://www.ijbiotech.com/article_229907_5ed569844e98cdaca3a8760fe1024c90.pdf
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