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Iranian Journal of Toxicology، جلد ۱۹، شماره ۴، صفحات ۰-۰
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عنوان انگلیسی Toxicity and fertility studies of Cornus capitata Well. fruit extract in Zebrafish Model
چکیده انگلیسی مقاله Background: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder, demanding the development of safe and effective treatment. Fruits of Cornus capitata Wall. are particularly known for their anticancer, antidiabetic, and antioxidant effects. This study aimed to evaluate the toxicity and therapeutic potential of the methanolic extract of Cornus capitata Well. fruits (MECCF) using fish embryo toxicity assay and testosterone-induced PCOS in zebrafish model.
Materials and Methods: Phytochemical screening and antioxidant activity (DPPH and Ferric reductase assay) of MECCF were evaluated. Embryo toxicity was evaluated in zebrafish embryos, on the basis of survival and morphological analyses using different concentrations of MECCF (5, 2.5, 1.25, 0.625, 0.3125 & 0.156 g/L). Adult zebrafish (3-8 months) were given testosterone (100ng) dissolved in distilled water containing 0.1% dimethyl sulfoxide followed by MECCF treatment (31.25 mg/L, 62.25 mg/L).
Results: In vitro antioxidant studies revealed a significant free radical scavenging property of MECCF. Embryo toxicity assay revealed that MECCF concentrations of 5, 2.5, 1.25, 0.625 g/L showed the highest rates of lethality. 0.3125, and 0.156 g/L were found to have no lethal effects even after 9 hours post-fertilization. Exposure to 62.25 mg/L MECCF in adult zebrafish showed significant changes in testosterone levels, gonado somatic index and ovarian health.
Conclusion: MECCF exhibits potent antioxidant activity and improves testosterone-induced PCOS symptoms in adult zebrafish, with low toxicity at appropriate concentrations in zebrafish embryos. Observed therapeutic effects may be attributed presence of triterpenoids and flavonoids, supporting its potential as a natural candidate for PCOS symptoms management.
کلیدواژه‌های انگلیسی مقاله Polycystic ovary syndrome, Zebrafish, Testosterone, Toxicity
نویسندگان مقاله | Indu Tewari
Pharmacology, School of Pharmaceutical Sciences, Sardar Bhagwan Singh University, Dehradun, Uttarakhand, India


| Veerma Ram
SBS UNIVERSITY, DEHRADUN


| Sanjay Singh
Siddhartha Institute of Pharmacy, Dehradun


| Mamta F Singh
College of Pharmacy, COER University, Roorkee, 247667, Uttarakhand.
نشانی اینترنتی http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1389-1&slc_lang=en&sid=1
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