Background: Polyphenolic compounds in buckwheat (Fagopyrum esculentum) are known for their antioxidant and detoxifying properties. Among them, rutin is a major flavonoid with potential biological activity. This study focused on the comparative analysis of polyphenolic content in roasted and green buckwheat and explored rutin’s interaction with sirtuin (SIRT), a key protein involved in cellular stress responses and longevity.
Materials and Methods: High-performance liquid chromatography (HPLC) was employed to quantify polyphenolic compounds in roasted and green buckwheat samples. In silico molecular docking was performed using CB-Dock2 to assess the binding affinity of rutin with the active site of SIRT.
Results: HPLC results showed that green buckwheat contained significantly higher levels of rutin compared to roasted buckwheat (1.09 mg/g vs. 0.5 mg/g). Molecular docking revealed a strong binding affinity between rutin and SIRT, supporting its potential role in modulating sirtuin-related protective pathways involved in detoxification.
Conclusion: Green buckwheat is a richer source of rutin, which may contribute to its enhanced detoxification potential. The strong in silico interaction between rutin and SIRT2  suggests that rutin could serve as a natural modulator of cellular defense mechanisms, particularly those regulated by sirtuins.