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Cell Journal، جلد ۱۷، شماره ۲، صفحات ۳۳۹-۳۵۴
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عنوان انگلیسی Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration
چکیده انگلیسی مقاله Objective: To investigate the transdifferentiation relationship between eight types of liver cell during rat liver regeneration (LR).
Materials and Methods: 114 healthy Sprague-Dawley (SD) rats were used in this experimental study. Eight types of liver cell were isolated and purified with percoll density gradient centrifugation and immunomagentic bead methods. Marker genes for eight types of cell were obtained by retrieving the relevant references and databases. Expression changes of markers for each cell of the eight cell types were measured using microarray. The relationships between the expression profiles of marker genes and transdifferentiation among liver cells were analyzed using bioinformatics. Liver cell transdifferentiation was predicted by comparing expression profiles of marker genes in different liver cells.
Results: During LR hepatocytes (HCs) not only express hepatic oval cells (HOC) markers (including PROM1, KRT14 and LY6E), but also express biliary epithelial cell (BEC) markers (including KRT7 and KRT19); BECs express both HOC markers (including GABRP,
PCNA and THY1) and HC markers such as CPS1, TAT, KRT8 and KRT18; both HC markers (KRT18, KRT8 and WT1) and BEC markers (KRT7 and KRT19) were detected in HOCs. Additionally, some HC markers were also significantly upregulated in hepatic stel-
late cells ( HSCs), sinusoidal endothelial cells (SECs) , Kupffer cells (KCs) and dendritic
cells (DCs), mainly at 6-72 hours post partial hepatectomy (PH).
Conclusion: Our findings indicate that there is a mutual transdifferentiation relationship between HC, BEC and HOC during LR, and a tendency for HSCs, SECs, KCs and DCs to transdifferentiate into HCs
کلیدواژه‌های انگلیسی مقاله Cell Transdifferentiation, Rat Liver Regeneration, Cell Isolation
نویسندگان مقاله Xiaguang Chen |
Animal Science and Technology School, Henan University of Science and Technology, Luoyang, China

Xu Cunshuan |
Key Laboratory for Cell Differentiation Regulation, Xinxiang, China;College of Life Science, Henan Normal University, Xinxiang, China

نشانی اینترنتی https://www.celljournal.org/article_250960_c8b28dfb8f0916aec20189754baef3a1.pdf
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