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Cell Journal، جلد ۱۸، شماره ۴، صفحات ۵۵۶-۵۶۴
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عنوان انگلیسی Melissa officinalis Acidic Fraction Protects Cultured Cerebellar Granule Neurons Against Beta Amyloid-Induced Apoptosis and Oxidative Stress
چکیده انگلیسی مقاله Objective: Extracellular deposition of the beta-amyloid (Aβ) peptide, which is the main finding in the pathophysiology of Alzheimer’s disease (AD), leads to oxidative damage and apoptosis in neurons. Melissa officinalis (M. officinalis) is a medicinal plant from the Lamiaceae family that has neuroprotective activity. In the present study we have investigated the protective effect of the acidic fraction of M. officinalis on Aβ-induced oxidative stress and apoptosis in cultured cerebellar granule neurons (CGN). Additionally, we investigated a possible role of the nicotinic receptor. Materials and methods: This study was an in vitro experimental study performed on mice cultured CGNs. CGNs were pre-incubated with different concentrations of the acidic fraction of M. officinalis for 24 hours, followed by incubation with Aβ for an additional 48 hours. CGNs were also pre-incubated with the acidic fraction of M. officinalis and mecamylamin, followed by incubation with Aβ. We used the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay to measure cell viability. Acetylcholinesterase (AChE) activity, reactive oxygen species (ROS) production, lipidperoxidation, and caspase- 3 activity were measured after incubation. Hochst/annexin V- fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining was performed to detect apoptotic cells. Results: The acidic fraction could protect CGNs from Aβ-induced cytotoxicity. Mecamylamine did not abolish the protective effect of the acidic fraction. AChE activity, ROS production, lipid peroxidation, and caspase-3 activity increased after Aβ incubation. Preincubation with the acidic fraction of M. officinalis ameliorated these factors and decreased the number of apoptotic cells. Conclusion: Our results indicated that the protective effect of the acidic fraction of M. officinalis was not mediated through nicotinic receptors. This fraction could protect CGNs through antioxidant and anti-apoptotic activities.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله ملیحه سودی | maliheh soodi


ابوالفضل دشتی | abolfazl dashti


هما حاجی مهدی پور | homa hajimehdipoor


شعله اکبری | shole akbari


نسیم عطایی | nasim ataei


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4722
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-367051.pdf
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