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Cell Journal، جلد ۱۸، شماره ۳، صفحات ۳۵۳-۳۶۱
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عنوان انگلیسی Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells
چکیده انگلیسی مقاله Objective: Micro-RNAs (miRNAs) are a class of posttranscriptional regulators that play crucial roles in various biological processes. Emerging evidence suggests a direct link between miRNAs and development of several diseases including type 2 diabetes (T2D). In this study, we aimed to investigate the effect of predicted miRNA and target genes on insulin resistance. Materials and methods: This experimental study was conducted on the C2C12 cell line. Using bioinformatics tools miRNA-135 and two respective target genes-insulin receptor (Insr) and vesicle associated membrane protein 2 (Vamp2)- were selected as potential factors involved in insulin resistance process. Levels of glucose uptake miRNA expression and respective gene targets were determined after cell transfaction by miR-135. Results: It was determined that Insr gene expression was significantly down-regulated in miR-135 transfected C2C12 cell line (P≤0.05). Interestingly; these transfected cells have shown a significant difference in glucose uptake incomparision the positive control cells, while it was similar to the insulin resistant cell line (P≤0.05). In contrast, no significant alteration of Vamp2 gene expression was observed. Conclusion: Our data indicated no change on the Vamp2 expression level after miRNA transfection, while expression level of Insr was reduced and miR-135 expression was contrarily increased leading to poor stimulation of glucose uptake through insulin, and development of insulin resistance phenotype in C2C12 cell line.
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نویسندگان مقاله مریم هنردوست | maryam honardoost


احسان عارفیان | ehsan arefian


مسعود سلیمانی | masoud soleimani


سارا صعودی | sara soudi


محمدرضا ساروخانی | mohammad reza sarookhani


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4563
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-367064.pdf
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