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Cell Journal، جلد ۱۸، شماره ۳، صفحات ۳۷۱-۳۸۰
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عنوان انگلیسی Upregulation of CD4+ T-Cell Derived MiR-223 in The Relapsing Phase of Multiple Sclerosis Patients
چکیده انگلیسی مقاله Objective: MicroRNAs (miRNA) are a class of non-coding RNAs which play key roles in post-transcriptional gene regulation. Previous studies indicate that miRNAs are dysregulated in patients with multiple sclerosis (MS). Th17 and regulatory T (Treg) cells are two subsets of CD4+ T-cells which have critical functions in the onset and progression of MS. The current study seeks to distinguish fluctuations in expression of CD4+ T-cell derived miR-223 during the relapsing-remitting (RR) phase of MS (RR-MS), as well as the expressions of Th17 and Treg cell markers. Materials and methods: This experimental study used real-time quantitative polymerase chain reaction (qRT-PCR) to evaluate CD4+ T cell derived miR-223 expression patterns in patients that experienced either of the RR-MS phases (n=40) compared to healthy controls (n=12), along with RNA markers for Th17 and Treg cells. We conducted flow cytometry analyses of forkhead box P3 (FOXP3) and RAR-related orphan receptor γt (RORγt) in CD4+ T-cells. Putative and validated targets of miR-223 were investigated in the miRWalk and miRTarBase databases, respectively. Results: miR-223 significantly upregulated in CD4+ T-cells during the relapsing phase of RR-MS compared to the remitting phase (P=0.000) and healthy individuals (P=0.036). Expression of RORγt, a master transcription factor of Th17, upregulated in the relapsing phase, whereas FOXP3 upregulated in the remitting phase. Additionally, potential targets of miR-223, STAT1, FORKHEAD BOX O (FOXO1) and FOXO3 were predicted by in silico studies. Conclusion: miR-223 may have a potential role in MS progression. Therefore, suppression of miR-223 can be proposed as an appropriate approach to control progression of the relapsing phase of MS.
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نویسندگان مقاله عارف حسینی | aref hosseini


کامران قایدی | kamran ghaedi


سمیه تنهایی | somayeh tanhaei


مزدک گنجعلی خانی حاکمی | mazdak ganjalikhani hakemi


شهره تیموری | shohreh teimuri


مسعود اعتمادی فر | masoud etemadifar


محمد حسین نصر اصفهانی | mohammad hossein nasr esfahani


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4565
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-367066.pdf
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