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Cell Journal، جلد ۱۸، شماره ۲، صفحات ۱۹۷-۲۰۴
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عنوان انگلیسی Overexpression of Annexin A1 Suppresses Pro-Inflammatory Factors in PC12 Cells Induced by 1-Methyl-4-Phenylpyridinium
چکیده انگلیسی مقاله Objective: Annexin A1 (ANXA1) is suggested to have anti-inflammatory function. However, the precise function of ANXA1 has remained unclear. In this study, we therefore examined the potency of ANXA1 in regulating reactive oxygen species (ROS) production and suppressing pro-inflammatory responses in PC12 cells induced by 1-methyl-4-phenylpyridinium (MPP+). Materials and methods: In this experimental study, cDNA of ANXA1 was cloned and inserted to the PGL268 pEpi-FGM18F vector to produce a recombinant PGL/ANXA1 vector for transfection into the PC12 cells. ANXA1 transfected cells were then treated with MPP+. Apoptosis and the content of pro-inflammatory factors including ROS, Interlukin-6 (IL-6), inducible nitric oxide synthase (iNOS) and nuclear factor-kappa B (NF-κB) were assessed by flow-cytometry, real-time quantitative polymerase chain reaction (RT-qPCR) and western blot in ANXA1-transfected cells and the data were compared with those obtained from mock and control cells. Results: Data revealed that overexpression of ANXA1 is associated with decreased levels of ROS and expression level of IL-6 and iNOS transcripts, and NF-κB protein in MPP+ treated PC12 cells. Conclusion: ANXA1 may be considered as an agent for prevention of neurodegenerative or inflammatory conditions.
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نویسندگان مقاله عباس کیانی اصفهانی | abbas kiani esfahani


صدیقه کاظمی sheykhshabani | sedigheh kazemi sheykhshabani


مریم پیمانی | maryam peymani


مطهره السادات هاشمی | motahare sadat hashemi


کامران قایدی | kamran ghaedi


محمد حسین نصر اصفهانی | mohammad hossein nasr esfahani


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4314
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-367085.pdf
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زبان مقاله منتشر شده en
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