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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۱۴-۱۵
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عنوان انگلیسی Is-15: Bioinspired Substrates Direct The Fate of Stem Cells
چکیده انگلیسی مقاله Objective: Bioinspired materials can mimic the stem cell environment and modulate stem cell differentiation and proliferation. In this context, biomaterials can mimic the biological microenvironments (i.e., niches) of stem cells and specifically affect the in vitro differentiation that is necessary for clinical application. In vivo, the appropriate differentiation, proliferation, and maintenance of potency are regulated by either stem cells or their specific niches. In this study, biomimetic micro/ nanoenvironments were fabricated by cell-imprinted substrates based on mature human keratinocyte morphological templates and also smart nanoenvironments were obtained by cell-imprinted substrates based on mature and dedifferentiated chondrocytes as templates. Materials and Methods :This substrate was characterized by SEM, AFM, Flouresent and Confocal microscopy. Toxicity of PDMS to stem cells was evaluated using an MTT-assay. The gene expression analysis of differentiated cells, were detected by Real Time PCR, array analysis and computer simulation study. Results: The data obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly (dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed (involucrin, collagen type I, and keratin 10) together with protein expression data showed that human adipose-derived stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape and characteristics of keratinocytes. The observed morphology of the ADSCs grown on the keratinocyte casts was noticeably different from that of stem cells cultivated on the stem-cell-imprinted substrates. Since the shape and geometry of the nucleus could potentially alter the gene expression, we used molecular dynamics to probe the effect of the confining geometry on the chain arrangement of simulated chromatin fibers in the nuclei. Additionally, rabbit adipose derived mesenchymal stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape (as determined in terms of cell morphology) and molecular characteristics (as determined in terms of gene expression) of the cell types which had been used as template for the cell-imprinting. Conclusion: The results obtained suggested that induction of mature cell shapes onto stem cells can influence nucleus deformation of the stem cells followed by regulation of target genes. This might pave the way for a reliable, efficient, and cheap approach of controlling stem cell differentiation toward human cells for wound healing applications.
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نویسندگان مقاله m محمودی | m mahmoudi


o ماشینچیان | o mashinchian


نشانی اینترنتی http://celljournal.org/journal/article/abstract/39
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