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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۱۵-۱۶
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عنوان انگلیسی Is-17: Rabbit Adipose Derived Mesenchymal Stem Cells for Bone Interface Regeneration
چکیده انگلیسی مقاله Adipose derived Mesenchymal Stem Cells (ADMSCs) have become a focus of research due to their multipotential properties. Adipose tissue is abundant in both humans and animals and can be easily harvested from subcutaneous tissue through percutaneous or limited open aspiration techniques and provides large volume of viable pluripotent stem cells. Adipose is the most profuse source of adult stem cells, and thus it holds great promise for use in tissue repair and regeneration and it shows fibroblastic morphology and characteristics. The objective of the present study is to evaluate the compatibility of tissue-engineered Hydroxyapatite-Burr Hole Button device (HAP-BHB) seeded with ADMSCs for bone regeneration. Burr-hole cranial neurosurgical procedures lead to the development of cosmetically puckered scars on the scalp over burr-hole sites. Ceramics, especially hydroxyapatite (HA) are good bone substitutes owing to their biocompatibility and osteo-conduction. The ADMSCs were cultured and maintained in DMEM-HG medium and are further characterized for the expression of MSCs surface markers CD44, CD90 and negative marker CD45. Confluent cells obtained after third passage were used for the study. Here, ADMSCs were seeded on to HAP-BHB and the cytotoxicity, oxidative stress response, apoptotic behavior, attachment, adherence were evaluated to predict efficacy of using ADMSC seeded Hydroxyapatite-Burr Hole Button device for bone interface regeneration The results of the 3-(4, 5- MTT dimethylthiazol)-2,5- diphenyl tetrazolium bromide) assay indicated that pow dered device material was non-cytotoxic up to (0.5 g/ ml) on cultured cells. It was also observed that oxidative stress related reactive oxygen species (ROS) production and apoptosis on cell seeded device was similar to that of control (cells alone) except in 3 days period, which showed an increased ROS generation. Scanning Electron and Confocal microscopy indicated a uniform attachment of cells and viability up to 200 nm deep inside the device. Based on the results, it can be concluded that the inhouse developed HAP-BHB device seeded with ADMSCs are non-toxic, compatible device for biomedical application and an attractive tissue engineered device for calvarial or similar defect regeneration.
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نویسندگان مقاله pv mohanan | pv mohanan


نشانی اینترنتی http://celljournal.org/journal/article/abstract/41
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