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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۱۷-۱۷
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عنوان انگلیسی Is-22: Photoreceptor Transplantation for The Restoration of Daylight Visual Responses in A Mouse Model of Cone Degeneration
چکیده انگلیسی مقاله Objective: Visual impairment and blindness due to the loss of photoreceptors represent one of the main causes for disability in industrialized societies. Currently no established therapy is available to rescue lost visual function. Cell replacement strategies by means of transplantation represent promising treatment approaches. Indeed, in recent years methods were developed to isolate, enrich and inject photoreceptors into animal models of retinal degeneration. However, the majority of photoreceptor transplantation studies so far used the mouse as a model system, thereby focusing on rod photoreceptors. Rods are functional in dim light conditions and therefore the nocturnal mouse retina is rod dominated. In contrast, human vision mainly depends on daylight vision provided by cone photoreceptors that also allow color detection. Here, we evaluated the potential of photoreceptor transplantation for daylight vision repair. Materials and Methods: As the mouse retina is a poor source for cones, we took advantage of cone-only retinas of neural retina leucine zipper-deficient (Nrl -/-) mice as a comprehensive source for transplantation studies. GFP-labeled Nrl -/- cone-like photoreceptors were enriched by CD73-based magnetic activated cell sorting and transplanted to the sub-retinal space of wild-type mice and a mouse model of cone degeneration, the cone photoreceptor function loss 1 (Cpfl1) mouse. 2, 4, 12 and 24 weeks post-transplantation experimental animals were investigated by immunohistochemistry, electron- microscopy and micro-electrode arrays (MEA). Results: Donor cone-like photoreceptors integrated into the outer nuclear layer of host wild-type and Cpfl1 retinas and generated a mature photoreceptor morphology including synapses and outer segments. Donor photoreceptors expressed cone-specific markers including s-opsin, cone arrestin and peanut agglutinin. Analysis by MEA showed stimulus-driven ON, OFF and ONOFF responses in retinal ganglion cells of Cpfl1 hosts under intensive light conditions. Conclusion: The transplantation of cone-like photoreceptors provided the proof-of-concept for the feasibility of daylight vision restoration upon cell transplantation in the mammalian retina.
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نویسندگان مقاله t سانتوز فریرا | t santos ferreira


k postel | k postel


h stutzki | h stutzki


g zeck | g zeck


m ader | m ader


نشانی اینترنتی http://celljournal.org/journal/article/abstract/46
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