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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۱-۲۱
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عنوان انگلیسی Ps-2: In Vitro Isolation, Culture and Identification of Human Endometrial Mesenchymal Stem/Stromal Cells (EnMSCs)
چکیده انگلیسی مقاله Objective: Dynamic remodeling of endometrium in each menstrual cycle (regeneration, differentiation, and shedding), for the purpose of its embryo implantation preparing, may be a reason on ability of endometrial basal layer cells for proliferation and differentiation into different cell types, that these functions are highly regulated by the stem cells. It has been hypothesized that adult stem/stromal cells are responsible for the cyclical regeneration during a woman’s reproductive phase. Medical application of stem cells could be for uterine-factor infertility which is caused by absence or dysfunction of the uterus in the future. Materials and Methods: Human endometrial tissues were collected from 3 cycling women after fully consent. Human endometrial tissues were scraped and dissociated into single-cell suspensions with mechanical and enzymatic digestion, prepared for cell culture. Cells were cultured in DMEM containing 10% FBS and incubated at 37ºC in 5% CO2. Endometrial mesenchymal stem/stromal cells (EnMSCs) were characterized by immuno-localisation with CD34, CD44, CD105, VIMENTIN, TRA-1-81, and TRA-2-54 markers. Results: EnMSCs were isolated, propagated (passaged more than seven times), frozen, thawed, and identified with specific marker for mesenchymal stem cell (CD105), hematopoietic stem cell marker (CD34), and mesenchymal stromal cell marker (VIMENTIN). However, adhesion marker (CD44), and human embryonic stem cell markers (TRA-1-81 and TRA-2-54) have not been expressed. Moreover, these cells were morphologically similar to mesenchymal stem cells (MSCs) in culture. Conclusion: Our preliminary data confirms other findings regarding isolation, culture and characterization of human EnMSCs. The endometrial tissue is an available source of MSCs acquirable with minimum side-effects that could be used for the future clinical applications for the patients with various types of uterine dysfunctions which can leads to recurrent pregnancy loss and finally resolve of surrogacy problems.
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نویسندگان مقاله f akyash | f akyash


a افلاطونیان | a aflatoonian


m رضازاده ولوجردی | m rezazadeh valojerdi


f صادقیان ندوشن | f sadeghian nodoushan


r افلاطونیان | r aflatoonian


b افلاطونیان | b aflatoonian


نشانی اینترنتی http://celljournal.org/journal/article/abstract/58
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