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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۱-۲۲
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عنوان انگلیسی Ps-3: Comparison of Human Osteoblast Proliferation in Alginate and Hydroxyapatite-Tricalcium Phosphate Scaffolds
چکیده انگلیسی مقاله Objective: After bone fractures from traffic trauma, many patients suffer from non-healing bone defects. So, it is important to identify modern and effective methods to improve healing of bone defects. One of them is, using bone cells from the patient himself, culture of these cells on appropriate scaffold and finally, transfer them to injured area.The main objective of this study is to compare the rate of osteoblast proliferation in alginate beads, and hydroxyapatite-tricalcium phosphate (HA-TC) scaffold. Materials and Methods: Bone tissue specimens were obtained from 4 patients undergoing craniotomy surgery. Bone specimens were cut in to small pieces and put in Petri dishes having culture medium. The cell cultures reached confluence, averagely after 2 weeks time. First passage cells were divided in two portions. One portion was used for hydroxyapatite-tricalcium phosphate scaffold, and the other was added to alginate gel. After a 2 weeks period, the data were collected and analyzed. Results: The osteoblasts in hydroxyapatite-tricalcium phosphate scaffold and alginate gel, had round morphology. Van kossa staining demonstrate, mineralized matrix in both groups. The number of harvested cells in 2 weeks after culture were significantly higher in Alginate group (P< 0.001). In addition MTT assay shows significant difference in the mean of viability rates between both groups in day 14 (P< 0.001). Conclusion: This study showed that Alginate gel support better proliferation and viability of osteoblasts in comparison with the hydroxyapatite-tricalcium phosphate scaffold. The probable cause of these differences can be searched in Alginate bio properties; porosity of Alginate gell provide conditions in which cellular and metabolic activities have been accelerated.
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نویسندگان مقاله f علی اکبری | f aliakbary


h بهرامیان | h bahramiyan


b هاشمی | b hashemi


s صادقی | s sadeghy


نشانی اینترنتی http://celljournal.org/journal/article/abstract/59
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