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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۲-۲۲
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عنوان انگلیسی Ps-4: Ly294002 Small Molecule Promote The Expression of Motor Neuron Genes
چکیده انگلیسی مقاله Objective: Phosphatidyl inositol 3-kinase (PI3K) is an enzyme implicated in signal transduction by associating with receptor and nonreceptor tyrosine kinases. PI3K/ Akt signaling has been implicated in multiple cellular and organ functions, including growth, cell survival, glucose metabolism and protein synthesis. Reports have shown that during neural differentiation, PI3K/Akt over-expression reduces glial (GFAP) and neuronal (btubulin III) marker expression and inhibits cell differentiation. It has been found that Ly294002 small molecule can inhibit PI3K/Akt pathway and promote neural differentiation. The human endometrial stem cells (hEn- SCs) are capable of extensive self-renewal and studies indicate that these cells have chondrogenic, osteogenic and neurogenic potential. The purpose of this study was to investigate the effect of Ly294002 small molecule on the expression of motor neuron genes. Materials and Methods: Endometrial biopsies were obtained from patients and then the cells were extracted enzymatically and cultured in DMEM/F12 medium supplemented by 10% FBS. The flow cytometry analysis was done for haemopoietic marker (CD34), mesenchymal stem cell markers (CD146, CD90 and CD105) and endothelial marker (CD31) in the third passage. In the first step of cell differentiation, FGF2, IBMX, B27 and 2ME was added to the media for one day. Then cells were treated with N2/B27 and Ly294002. After 3 days, Retinoic acid (RA) and Ly294002 was added to the media for 8 days. At the last, cells were treated with N2/B27 and BDNF for other 7 days. The mRNA levels of several genes were measured including NF, Nestin, chat and GFAP by Real-time PCR Technique. Results: Real-time PCR data showed that mRNA levels of NF, Nestin, chat and GFAP were increased in LY294002 treated hEnSCs. Conclusion: Our data suggest that Ly294002 can promote the neuronal fate decision in hEnSCs through promoting the expression of motor neuron genes and inhibition of the PI3K/AKT pathway.
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نویسندگان مقاله h عنبر | h anbar


h محسنی کوچصفهانی | h mohseni kouchesfehani


s ابراهیمی باروق | s ebrahimi barough


| a rahimi


| a rahimi


نشانی اینترنتی http://celljournal.org/journal/article/abstract/60
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