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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۲-۲۳
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عنوان انگلیسی Ps-5: A Useful Method of Platelet Rich Plasma (PRP) Isolation and Preparation in Human
چکیده انگلیسی مقاله Objective: To date, platelet-rich plasma (PRP) is widely applied in medical treatment as a pool of different growth factors such as PDGF, IGF, TGF, BGGF, and BDNF. This worthy component is used for improving tissue regeneration in healing therapies, plastic surgery and orthopedics. So, we reported an affected PRP isolation protocol to standardize a methodology for autologous platelet rich plasma in human. Materials and Methods: 15 ml peripheral blood from six healthy donors (male- between 20- 30 years old) was collected. Mechanical procedures such as fast pipetting, strong shaking can activate platelets and should be noticed. At first, freshly collected blood was centrifuged for 5 min at 280×g and RT to reduce the number of red and white blood cells. At second, centrifugation was done at 450×g for 12 minutes at RT to settle platelets. PRP samples were counted by the Neubauer chamber compared to peripheral blood platelets as a control group. Results: We recovered nearly 4 to 6 folds increase in platelet concentration (1.2×106 to 1.9×106 platelets/μl). The concentration of red/ white blood cells was only < 1% at final PRP preparation. Conclusion: PRP has been recently considered as a growth factor source in different clinical therapies. One of the most negatively important factors at this component is blood-derived cell types that should be eliminated from PRP because of its potential proinflammatory effect. Reduced concentration of leukocyte is one the advantages of this protocol. Less time to perform platelet isolation can also improve the PRP preparation procedure.
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نویسندگان مقاله a عاطفی | a atefi


a shoaraye نجاتی | a shoaraye nejati


mh بیگی | mh beigi


mh نصر اصفهانی | mh nasr esfahani


نشانی اینترنتی http://celljournal.org/journal/article/abstract/61
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