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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۳-۲۴
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عنوان انگلیسی Ps-7: A Novel Electroactive Polyurethane Scaffold for Cardiac Tissue Engineering
چکیده انگلیسی مقاله Objective: Our aim was to investigate the potential of electroactive polyurethane scaffold for cardiac tissue engineering. Materials and Methods: A novel conductive polyurethane containing aniline pentamer (AP) was blended with polycaprolactone (PCL) and further fabricated into scaffolds using double porogen particulate leaching and compression molding method. Physico-chemical characterizations of the blend scaffold (PB) were evaluated by scanning electron microscope, porosity measurements, mechanical analysis and electrical conductivity measurements. For cell evaluations, cardiomyocytes were isolated from 1-2 days old newborn Wistar rats. Cell seeded scaffolds were assessed by immunofluorescent staining for comparative evaluation of cardiomyocyte specific proteins, including troponin T (TrpT) and connexin43 (Conx43) .Gene expression profiles of the cardiac markers including TrpT-2, Conx43 and Actinin alpha4 (Actn4) were analyzed by quantitative real-time PCR. All the results were compared with the non conductive PCL scaffold. Results: Our results showed a consistent porous morphology all through both scaffolds with the interconnected pores throughout the whole structure ranging from several μm to 150 μm. PB scaffold had compression modulus and strength of 4.1 and 1.3 MPa, respectively. The conductivity of the scaffold was measuredas 10-5 ± 0.09 S/cm and preserved in culture medium for at least 100 h post fabrication. Scaffolds also supported neonatal cardiomyocytes adhesion and growth. Incorporation of AP led to the increased expression of the cardiac genes involved in muscle contraction and relaxation (troponin-T) and cytoskeleton alignment (actinin- 4). Conclusion: Our study highlighted the potential of embedding the electroactive moiety in the structure of the scaffold to direct electrical signals on cell activities for tissue engineering applications. Our data demonstrated that the inherently electrical conductive substrates are non-toxic and support cell proliferation and attachment.
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نویسندگان مقاله n بحیرایی | n baheiraei


h یگانه | h yeganeh


r غریبی | r gharibi


s وحدت | s vahdat


نشانی اینترنتی http://celljournal.org/journal/article/abstract/63
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