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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۴-۲۴
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عنوان انگلیسی Ps-8: Effects of Scaffold Architecture on Efficiency of Mechanical Stimulations of Mesenchymal Stem Cells Under Fluid Flow
چکیده انگلیسی مقاله Objective: Mesenchymal stem cells (MSCs) are considered as multipotent stem cells which is mainly presented in bone marrow. Combining these cells with tissue engineering scaffolds is a promising method to regenerate tissues and organs. MSCs can be readily affected by cell culture conditions and biophysical stimuli. A dynamic culture -provided by a bioreactor, induces various mechanical stimuli on MSCs and in a long term- can influence proliferation, differentiation and cell fate determination. This investigation offers a finite element approach to define biophysical stimuli such as fluid shear stress and hydrodynamic pressure on cellular level and consequently the results can be used to predict cell fate as well. Materials and Methods: In this study, using computeraided design tools, two different scaffold architectures -based on implicit surfaces- were modeled. The single cell assuming the MSCs mechanical properties with idealized geometry was designed and attached in different positions on the scaffolds. Using tools of computational fluid dynamics and employing 1.2 million tetrahedral and prism elements, inlet fluid velocities of 50 and 100 μm/s were applied to simulate dynamic culture. Further, the solution satisfied mesh independency and accuracy. Results: The results disclosed that a change in single cell position altered the magnitudes of shear stresses and hydrodynamic pressures. Furthermore, shift in scaffold architectures resulted in different fluid shear stress and hydrodynamic patterns on the single cell. Based on the results, the scaffold perpendicular channels with respect to fluid flow provided adequate conditions for cell culture. The models specified distribution of effective shear stresses and hydrodynamic pressure on both scaffolds and the single cell. Conclusion: Current study showed that in particular fluid flow, there are specific surfaces in every scaffold transmitting optimal mechanical stimuli. Considering these crucial factors help researchers to modify the dynamic culture systems in order to set up more functional experiments in vitro.
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نویسندگان مقاله a بخشیان نیک | a bakhshian nik


b وحیدی | b vahidi


m مرادخانی | m moradkhani


نشانی اینترنتی http://celljournal.org/journal/article/abstract/64
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