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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۴-۲۴
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عنوان انگلیسی Ps-9: A Rapid, Simple and Economical Method for The Isolation of Mesenchymal Stem Cells from Wharton’s Jelly by Phosphate Buffer Saline
چکیده انگلیسی مقاله Objective: The isolation of mesenchymal stem cells (MSCs) from the Wharton�s jelly (WJ) without degradation of cellular surface receptors and protection cellular function, proliferation and viability is important for research and clinical applications. In this study we have established a simple, rapid protocol without enzymatic treatment in less time to isolate MSCs from WJ. Materials and Methods: Human umbilical cords were collected after full-term deliveries and transported to the laboratory in sterile phosphate-buffered saline (PBS). Umbilical cords were cut into segments of 1�2 cm and removed cords vessels. The WJ sectioned were transferred into PBS and put on a shaker for 2 hours. The cord segments were discarded and the suspension was centrifuged and cultured in DMEM-LG supplemented with 10% fetal bovine serum for 5 days. The plastic adherent cells were investigated for surface markers CD90, CD105, CD73, CD44, HLA-DR, CD34/45, and CD133. Adipogenic, osteogenic, and chondrogenic differentiations were performed to investigate mesenchymal nature. Results: After 5 days, purified populations of spindleshape MSCs were appeared and the cells then expanded until they reached subconfluence . The expanded cells were positive for CD90, CD105, CD73, CD44 , and negative for CD34/45, CD133 and HLA-DR surface markers. WJ-MSCs showed multilineage cell differentiation potential into adipogenic, osteogenic and chondrogenic phenotypes. Conclusion: This study indicates that this non-enzymatic protocol can result in efficient isolation of MSCs from WJ with less time. The expanded cells expressed characteristic markers and presented typical functional properties of MSCs such as differentiation capacities.
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نویسندگان مقاله b بیکی | b beiki


m ضرابی | m zarrabi


m رادمنش | m radmanesh


نشانی اینترنتی http://celljournal.org/journal/article/abstract/65
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