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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۴-۲۵
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عنوان انگلیسی Ps-10: Effect of Digoxin on Mesenchymal Stem Cell Proliferation and Apoptosis.
چکیده انگلیسی مقاله Objective: Cardiac glycosides -such as digoxin or digitoxin- are the natural products that traditionally used to increase cardiac contractile force in patients with heart failure and cardiac arrhythmia. It has been shown that digoxin can directly inhibit the cell proliferation and lead to cell apoptosis. Present study was conducted to analyze the effect of digoxin in the cohorts of mesenchymal stem cells (MSCs) -based therapy. Materials and Methods: MSCs were cultured and treated with different concentrations (0.1, 0.5, 1, 5, 7, 10, 15, 20, 30 and 40 μM) of digoxin for 6, 12, 24 and 48 hours. MTT assay was performed for cell viability and proliferation study. Also, to evaluate cell apoptosis a TUNEL assay was conducted. Results: After 24 and 48 hours, cell viability was significantly decreased in 20, 30 and 40 μM concentrations (P< 0.05), but significant decline in cell viability was observed only in 40 μM after 12 hours (P< 0.05). After 6 hours, cell viability of experimental groups had no significant differences toward control group (P>0.05). In addition, cell apoptosis was increased along with rise in drug concentration and time. After 24 and 48 hours, cell apoptosis in 10 μM, 20 μM, 30 μM and 40 μM concentrations were higher than control group, significantly (P< 0.05). Conclusion: According to our obtained results we suggest that digoxin may lead to decline in cell survival ability and increase in cell apoptosis in a dose/time dependent pattern.
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نویسندگان مقاله sh دارابی | sh darabi


h رفیقدوست | h rafighdoost


mh انصاری | mh ansari


a محمدی روشنده | a mohammadi roushandeh


نشانی اینترنتی http://celljournal.org/journal/article/abstract/66
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