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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۵-۲۵
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عنوان انگلیسی Ps-11: Nanofiber-Expanded Human Umbilical Cord Blood-Derived CD34+ Cell Therapy Accelerates Murine Cutaneous Wound Closure by Attenuating Pro-Inflammatory Factors And Secreting IL-10
چکیده انگلیسی مقاله Objective: Nanofiber-expanded human umbilical cord blood-derived CD34+ cell therapy is under consideration for treating peripheral and cardiac ischemia. However, the therapeutic efficacy of nanofiber-expanded human umbilical cord blood-derived (NEHUCB) CD34+ cell therapy for wound healing and its mechanisms are yet to be established. Materials and Methods: Human umbilical cord blood stem cells were isolated from freshly collected samples, and were expanded using novel nanofiber mediated expansion technology. Expanded cells characteristics were determined by flowcytometry. Cuteneous wound were developed in immunocompromised NOD/SCID mice. Nanofiber-expanded CD34+ stem cells were injected to the mice using lateral tail vein and healing were measured. Cytoking and growth factor expression was measured by Real-time PCR methods from wound tissues. Histological analysis were performed to determine wound healing mechanisms. Finally mechanism confirmed using in vitro methods with human primary fibrobalst cells and stem cells. Results: Using an excision wound model in NOD/ SCID mice, we show herein that NEHUCB-CD34+ cells home to the wound site and significantly accelerate the wound-healing process compared to vehicle- treated control. Histological analysis reveals that accelerated wound closure is associated with the re-epithelialization and increased angiogenesis. Additionally, NEHUCB-CD34+ cell-therapy decreases expression of pro-inflammatory cytokines, such as TNF-�, IL-1�, IL-6 and NOS2A in the wound bed, and concomitantly increased expression of IL-10 compared to vehicletreated control. These findings were recapitulated in vitro using primary dermal fibroblasts and NEHUCBCD34+ cells. Moreover, NEHUCB-CD34+ cells attenuate NF-�B activation and nuclear translocation in dermal fibroblasts through enhanced secretion of IL-10, which is known to bind to NF-�B and suppress transcriptional activity. Conclusion: Collectively, these data provide novel mechanistic evidence of NEHUCB-CD34+ cell-mediated accelerated wound healing.,"dai
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نویسندگان مقاله | m dastpak


نشانی اینترنتی http://celljournal.org/journal/article/abstract/67
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