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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۵-۲۶
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عنوان انگلیسی Ps-12: Investigating The Behavior of A Dual Promoter System Containing Spermatogonial Specific Genes in The Presence of Extrinsic Factors
چکیده انگلیسی مقاله Objective: Germ cells are responsible for the origin of new organisms and guarantee genetic continuity across the generations. Spermatogonial stem cells (SSCs) are a type of germ cells with unlimited proliferative poten tial. Their pluripotency and lower ethical concerns have made them the most suitable candidates for biotechnological, medicinal and transgenic studies as a substitute for embryonic stem cells. Materials and Methods: In this study, we designed a dual promoter based lentiviral vector in order to track the simultaneous activity of Stra8 and c-kit promoters monitored by expression analysis of their downstream reporter genes (ZsGreen and DsRed2, respectively). The functional activity of promoters engineered in this construct was evaluated by transfecting GC-1 spg germ cell line when cells were under-treatment with RA, testis extract and CHIR99021 (a reprograming factor) using flow cytometry and real-time PCR approaches. Results: We observed a different dynamic behavior of both promoters in the presence of extrinsic factors. The efficiency and accuracy of construct promoters in the presence of CHIR99021 were also analyzed and found to be comparable to the genomic endogenous promoters. Conclusion: Our designed vector with the advantage of having all the genetic regulatory elements in a unique construct, could report the genetic responses to the exogenous stimuli. Further studies regarding multiple promoters expression would be required to engineer lentiviral vectors as functional tools for tracking the developmental events in germ cells.
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نویسندگان مقاله | m dastpak


m متین | m matin


m frashchian | m frashchian


m irfanmaqsood | m irfanmaqsood


s siskhatnezhad | s siskhatnezhad


ar بهرامی | ar bahrami


نشانی اینترنتی http://celljournal.org/journal/article/abstract/68
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