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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۶-۲۶
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عنوان انگلیسی Ps-13: Purification and Transplantation of Human Embryonic Stem Cell Derived Midbrain Dopaminergic Progenitor Cells into The Rat Model of Parkinson's Disease
چکیده انگلیسی مقاله Objective: Human embryonic stem cells (hESCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy in Parkinson’s disease. Materials and Methods: In this study we used a defined protocol to generate human DA progenitor cells from human embryonic stem cells that is based on dual SMAD inhibition and embryoid body (EB) formation. Results: Our results showed that the human DA progenitor cells expressed the midbrain DA progenitor markers,FOXA2, CORIN, LMX1A and LMX1B and can be successfully differentiate into TH+ mature neurons. We surveyed the proteome of the human DA progenitor cells for novel surface proteins in previous study which resulted in expression of CNTN2, Flot2 and Calretinin. Flowcytometry analysis of these cells indicated that they express 20% CNTN2, 17% Flot2, 11% Calretinin and 22% Corin. To evaluate the functionality of these cells, we transplanted CNTN2+ cells in day 12 post neural induction into 6-OHDA-lesioned rats and also transplanted unsorted DA progenitor cells and human dermal fibroblasts (HDF) as control. The behavioral results of motor performance tests, such as apomorphine-induced rotation and cylinder test, showed that CNTN2+ cells can significantly improve motor behavior from week 2, compared to unsorted cells. Conclusion: Our method is favorable in terms of efficiency and safety indicating promise approach for the development of cell-based therapies in Parkinson’s disease.
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نویسندگان مقاله b دولت یار | b dolatyar


a فتحی | a fathi


s صحرایی | s sahraei


gh حسینی salekdeh | gh hosseini salekdeh


h بهاروند | h baharvand


m جوان | m javan


نشانی اینترنتی http://celljournal.org/journal/article/abstract/69
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