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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۶-۲۷
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عنوان انگلیسی Ps-14: The Role of bFGF, BMP4 and Noggin in Cardiomyocyte Differentiation of Human Adipose Tissue-Derived Stem Cells
چکیده انگلیسی مقاله Objective: The aim of the present study was to evaluate the role of bFGF, BMP4 and noggin on cardiomyocyte differentiation from human adipose tissue-derived stem cells (ADSCs). Materials and Methods: Human ADSCs were isolated from the adipose tissue samples of patients who underwent abdominoplasty. ADSCs were characterized by flow cytometry analysis. Third-passaged ADSCs were cultured to reach 100% confluency. Cardiac differentiation was induced by 4-day incubation of the ADSCs in DMEM plus 10% fetal bovine serum (FBS) or B27 (1X), 10 ng/ml bFGF and 10, 20 or 50 ng/ml BMP- 4. After this initial stage, differentiation was continued in FBS-containing medium, and cardiac differentiation was examined after 21 days. The expression of cardiac transcription factors and cardiac-specific genes was evaluated by RT-PCR, quantitative real-time PCR, immunocytochemistry, flow cytometry and western blot analyses. Results: Three-week differentiated ADSCs showed the expression of cardiac transcription factors, GATA4 and MEF2C, and cardiac-specific genes, α-MHC, β-MHC, MLC2V, MLC2A and ANP. The expression of MLC2V and ANP mRNAs were at their highest level when the initial phase of differentiation was performed in FBScontaining DMEM and at the presence of 10 ng/ml bFGF and 20 ng/ml BMP-4. Immunocytochemical analyses showed the expression of α-actinin and cardiac troponin I proteins in the ADSC-derived cardiomyocytes. As revealed by flow cytometry analysis, a significant proportion of the differentiated cells were stained positively for cardiac troponin I, and western blot analysis confirmed the expression of Connexin43, α-actinin and Desmin proteins. Conclusion: The results of present study indicate an important role for bFGF and BMP-4 in cardiomyocyte differentiation from ADSCs. Moreover, cardiac differentiation was more effective when the initial phase of differentiation was performed in FBS-containing medium.
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نویسندگان مقاله m فخرطه | m fakhr taha


s حسنی | s hassani


a جاوری | a javeri


نشانی اینترنتی http://celljournal.org/journal/article/abstract/70
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