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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۶-۲۶
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عنوان انگلیسی Ps-15: The Effect of Hypoxia during Differentiation of Human Embryonic Stem Cell Spheres to Hepatospheres in Spinner Flask
چکیده انگلیسی مقاله Objective: Integrated scale up production of hepatocytes from hESC spheres (hESpheres) in spinner flask will facilitate the application of hepatocytes in drug screening assays and in bioartificial livers. But there are some problems in spinner flask including its large volume and low surface area which can lead to reduction in dissolved oxygen and therefore hypoxia will increase in this condition which can affect the efficient differentiation of hESpheres to hepatocytes spheres (hepatospheres). In this study, we explored the role of hypoxia in comparison with normoxia during differentiation of hESpheres to hepatospheres in suspension dynamic culture. Materials and Methods: hESpheres by adding different growth factors were differentiated to hepatospheres in spinner flask. The morphology, gene and protein expression of differentiated cells were compared under normoxia and hypoxia conditions. Results: The cystic hepatospheres increased under hypoxia condition (30%) compared to normoxia (8%). The total sizes of hepatospheres under hypoxia condition were smaller than normoxia. Hepatospheres with ALBpositive cells under hypoxia was less than normoxia condition (47 and 75%, respectively). The expression of AFP and AAT as early markers in hepatospheres, under hypoxia was more than normoxia condition. Conclusion: Results showed that hypoxia reduces the efficiency of hepatocytes production from hESpheres in spinner flask. To achieve efficient integrated large scale differentiation of hESpheres to hepatospheres, it is necessary to develop large scale differentiation in computer-controlled bioreactor instead of uncontrolled spinner flasks. Computer-controlled bioreactors may help to produce high quality hepatocytes with appropriate quantity for drug screening assays or bioartificial livers.
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نویسندگان مقاله z فرزانه | z farzaneh


m نجاراصل | m najarasl


m رضایی لاریجانی | m rezaii larijani


h بهاروند | h baharvand


نشانی اینترنتی http://celljournal.org/journal/article/abstract/71
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