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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۷-۲۸
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عنوان انگلیسی Ps-16: Simple, Efficient and Cost-Benefit Protocol for Differentiation of Human Embryonic Stem Cell Spheres to Endodermal Cells
چکیده انگلیسی مقاله Objective: Endoderm lineage is one of the important layers during human embryogenesis. Most cells in gut tube and its appendix -such as liver and pancreas- are derived from endodermal cells. Efficient and large scale production of endodermal cells as well as their derivation in-vitro are very important for drug screening assays, proteome analysis, artificial organ development in-vitro and animal model assessments. Therefore, development of a high efficiency and cost-benefit protocol for scale up production of these cells from human embryonic stem cells (hESCs) is very essential. In this study for the first time, we developed a new simple, efficient and cost-benefit protocol for large scale production of endodermal cells from hESCs in suspension culture. Materials and Methods: Pluripotent hESpheres were differentiated to mesoendodermal cells at the first step, then to endodermal cells. During activin treatment, the concentration of this component and competency of endodermal cells for differentiation to hepatocytes were evaluated. We also assessed the endodermal cells and hepatocytes production potential of this new protocol at large scale in spinner flask. Results: In this study, it has been shown when hESpheres pretreated with high dose of WNT agonist, CHIR, followed by short term treatment -two days- in low concentration of activin; they were differentiated efficiently to endodermal cells in both static and dynamic suspension culture. Conclusion: Pretreatment of hESpheres with high WNT agonist dose followed by low activin treatment was sufficient for differentiation of hESCs to endodermal cells. The endodermal cells produced by this new protocol are useful tools for scale up production of hepatocytes or B-cells for other studies.
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نویسندگان مقاله z فرزانه | z farzaneh


h انصاری | h ansari


m نجاراصل | m najarasl


h بهاروند | h baharvand


نشانی اینترنتی http://celljournal.org/journal/article/abstract/72
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