این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۸-۲۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-17: The Effect of bFGF and Fetal Calf Serum on Proliferation of Chicken Primordial Germ Cells and Stromal Cells
چکیده انگلیسی مقاله Objective: Chicken gonadal tissue has two cell populations, incuding primordial germ cells (PGCs) and stromal cells (somatic cells). PGCs are suitable source for production of chicken pluripotent stem cell lines in transgenic birds, and subsequently vaccine and recombinant protein productions. In general, the effect of growth factors such bFGF, mLIF and serum for proliferative capacity in vitro are clear and important. Materials and Methods: After incubation of fertilized chicken egg up to 6 days and isolation of primary gonadal tissues and culturing mixture of the cells -PGCs and stromal cells- these somatic cells proliferate in present of fetal calf serum (FCS) and bFGF, as two important factors for PGC culture. Somatic cells produce a multilayer feeder under the PGCs in primary culture and PGCs make a small cluster under these cells. Results: In presence of growth factor and high volume of FCS (15%), only up to 4 or 5 days PGCs could be observed; while after that the stromal cells proliferate more than PGCs and cause PGCs disappearance. Conclusion: Until now, we have seen many researches about derivation and maintenance of chicken PGCs, with hopes to understand the mechanisms that occur during germline development and produce therapeutic agents by transgenic birds. There are still many unknown questions in this area and this project will try to have efficient conditions for identification of suitable culture medium for long term culture of PGCs in vitro without serum and feeder cells.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله m فرزانه | m farzaneh


sn حسنی | sn hassani


h بهاروند | h baharvand


نشانی اینترنتی http://celljournal.org/journal/article/abstract/73
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات